To allow a more rapid determination of heart-type fatty acid-binding protein (FABP) concentration in plasma a direct non-competitive (sandwich-type) ELISA was developed which uses high-affinity monoclonal antibodies to FABP. Total performance time of the one-step immunoassay is 45min. The standard curve was linear between 0·2–6μg/L, and the within-run and between-run coefficients of variations were below 6 and 11%, respectively. The serum FABP concentration measured in 79 healthy individuals was 1·6 (0·8) [mean (SD), range 0·3–5·0] μg/L. The assay can be used for rapid plasma or serum FABP measurement in the early diagnosis of acute myocardial infarction.
GlatzJFCVan BilsenMPaulussenRJAVeerkampJHVan der VusseGJRenemanRS. Release of fatty acid-binding protein from isolated rat heart subjected to ischemia and reperfusion or to the calcium paradox. Biochim Biophys Acta1988; 961: 148–52
2.
KnowltonAABurnerREBrecherP. Rabbit heart fatty acid-binding protein: Isolation, characterization, and application of a monoclonal antibody. Circ Res1989; 65: 981–8
3.
TanakaTHirotaYSohmiyaKNishimuraSKawamuraK. Serum and urinary human heart fatty acid-binding protein in acute myocardial infarction. Clin Biochem1991; 24: 195–201
4.
AdamsJEAbendscheinDRJaffeAS. Biochemical markers of myocardial injury. Is MB creatine kinase the choice for the 1990s?Circulation1993; 88: 750–63
5.
KleineAHGlatzJFCVan NieuwenhovenFAVan der VusseGJ. Release of heart fatty acid-binding protein into plasma after acute myocardial infarction in man. Mol Cell Biochem1992; 116: 155–62
6.
TsujiRTanakaTSohmiyaKHirotaYYoshimotoKKinoshitaK. Human heart-type cytoplasmic fatty acid-binding protein in serum and urine during hyperacute myocardial infarction. Int J Cardiol1993; 41: 209–17
7.
Van NieuwenhovenFAKleineAHWodzigKWHHermensWTKragtenJAMaessenJG. Discrimination between myocardial and skeletal muscle injury by assessment of the plasma ratio of myoglobin over fatty acid-binding protein. Circulation1995; 92: 2848–54
8.
GlatzJFCKleineAHVan NieuwenhovenFAHermensWTVan Dieijen-VisserMPVan der VusseGJ. Fatty acid-binding protein as a plasma marker for the estimation of myocardial infarct size in humans. Br Heart J1994; 71: 135–40
9.
OhkaruYAsayamaKIshiiHNishimuraSSunaharaNTanakaT. Development of a sandwich enzyme-linked immunosorbent assay for the determination of human heart type fatty acid-binding protein in plasma and urine by using two different monoclonal antibodies specific for human heart fatty acid-binding protein. J Immunol Meth1995; 178: 99–111
10.
KragtenJAHermensWTVan Dieijen-VisserMP. Quantitation of cardiac troponin T release into plasma after acute myocardial infarction. Comparison with enzyme release. Ann Clin Biochem1996; 33: 314–23
11.
RoosWEymannESymannekMDuppenthalerJWodzigKWHPelsersMMALGlatzJFC. Monoclonal antibodies to human heart fatty acid-binding protein. J Immunol Meth1995; 183: 149–53
12.
BlandJMAltmanDG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet1986; i: 307–10
RabkinRDahlDO. Renal uptake and disposal of proteins and peptides. In: AudusKLRaubTJ, eds. Biological Barriers To Protein Delivery.New York: Plenum Press, 1993: 299–338
15.
ChenI-WDavidRMaxonHRSperlingMSteinEA. Age-, sex-, and race-related differences in myoglobin concentrations in the serum of healthy persons. Clin Chem1980; 26: 1864–8
16.
ChapelleJPLemacheKEl AllafMEl AllafDPiérardL. Fast determination of myoglobin in serum using a new radial partition immunoassay. Clin Biochem1994; 27: 423–8
17.
BrothersGCardoneBMangionJStybaGJackowskiGShaikhN. Development and characterization of monoclonal and polyclonal anti-bodies to human heart fatty acid-binding proteins [Abstract]. Clin Chem1995; 41: S88
18.
Siegmann-ThossCRennebergRGlatzJFCSpenerF. Enzyme immunosensor for diagnosis of myocardial infarction. Sensors Actuators1996; B 30: 71–6
