A high-performance liquid chromatography (HPLC) method for the determination of sweat chloride was developed and evaluated. This method is capable of measuring chloride ion in sweat eluates with an analytical working range from 0·5 mmol/L to 6 mmol/L (15 to 180 mmol/L in undiluted sweat samples). Precision studies showed that at the levels of 1 mmol/L, 2·5 mmol/L and 3·5 mmol/L, the within batch coefficients of variation were 1·26%, 0·88% and 0·43%, respectively, and the between batch coefficients of variation were 1·31%, 1·75% and 1·07%, respectively. The minimum detection limit was 0·5 mmol/L. This method correlated well with the ion-selective electrode (ISE) method currently in use.
WebbAKDavidTJ. Clinical management of children and adults with cystic fibrosis. BMJ1994; 308: 459–62.
2.
BarbourHM. Development and evaluation of the simultaneous determination of sweat sodium and chloride by ion selective electrodes. Ann Clin Biochem1991; 28: 150–4.
3.
WarwickWJHansenLVielaIMathesonJ.Comparison of the chloride electrode and gravimetric chloride titration sweat tests. Am J Clin Pathol1977; 72: 142–5.
4.
GibsonLECookeRE. Test for concentations of electrolytes in sweat in cystic fibrosis of pancreas utilising pilocarpine by iontophoresis. Pediatrics1959; 23: 545–9.
5.
SmallHStevensTSBaumanWC. Novel ion exchange chromatographic method using conductometric detection. Anal Chem1975; 47: 1801.
6.
PohlCAHohnsonEL. Ion chromatography—the state of the art. J Chromatogr Sci1980; 18: 442–52.
7.
BidlingmeyerBA. Separation of ionic compounds by reversed phase liquid chromatography: An update of ion pairing techniques. J Chromatogr Sci1980; 18: 525–39.
8.
RiedmanMGlatzB.Sensitive HPLC analysis of inorganic anions using standard equipment. Int Chromatogr Lab1994; 22: 7–14.
9.
BlandJMAltmanDG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet1986; i: 307–10.
