The study of proteinuria, and especially the search for Bence Jones proteins (BJP), has almost always required urine concentration. We evaluated a high-sensitivity electrophoretic method using unconcentrated urine based on colloidal gold staining. The sensitivity for the detection of BJP is further enhanced by immunofixation. Sensitivity to BJP is better than 1 mg/L and, apart from the α-1 microglobulin, all the proteins relevant to the classification of proteinuria can be visualized with a sensitivity of approximatively 3 mg/L.
NuxbaumJChubaJVHellmanGCSolomonAGalloGR. Monoclonal immunoglobulin deposition disease: Light chain and light and heavy chain deposition diseases and their relation to light chain amyloidosis. Ann Int Med1990; 112: 455–64.
7.
BhagavanNVCodyLScottoliniAG. Screening method for detection of Bence-Jones proteinuria. Clin Chem1988; 34: 171.
8.
BoegeFKoehlerBLiebermannF. Identification and quantification of Bence-Jones proteinuria by automated nephelometric screening. J Clin Chem Clin Biochem1990; 28: 37–42.
9.
MacNamaraEMAguzziFPetriniCRestricted electrophoretic heterogeneity of immunoglobulin light chains in urine. A cause for confusion with Bence Jones protein. Clin Chem1991; 37: 1570–4.
10.
BellottiVMerliniGBucciarelliEPerfettiVQuagliniSAscariE. Relevance of class, molecular weight and isoelectric point in predicting human light chain amyloidogenicity. Br J Haematol1990; 74: 65–9.
MartinSMKohnJ. The sensitivity of gold staining in the detection of Bence Jones proteinuria (abstract). Ann Clin Biochem1987; 24 (suppl 1): 120–1.
13.
GasparroCDolciABoniniPAAguzziF. Gold staining in the detection and typing of Bence Jones proteinuria (Abstract). Bioc Clin1989; 13 (suppl 18): 317.
14.
WhicherJTHawkinsLHigginsonJ. Clinical application of immunofixation: A more sensitive technique for the detection of Bence Jones protein. J Clin Pat1980; 33: 779–80.
15.
WallerKVWardKMMahanCWismattDK. Current concepts in proteinuria. Clin Chem1989; 35: 755–65.
16.
StierleHEOserBBoeskenWH. Improved classification of proteinuria by semiautomated ultrathin SDS polyacrylamid gel electrophoresis. Clin Nephr1990; 4: 168–73.
17.
AguzziFPetriniCGasparroC. Attività COM.05 Indagine Etf 1988. Biochim Clin1989; 13: 447–50.
18.
AguzziFRezzaniA. An advantageous but neglected technique for immunofixation of monoclonal components on cellulose acetate membranes. Standardisation and evaluation. Giorn It Chim Clin1986; 11: 293–9.
19.
BradfordMA. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem1976; 72: 248.
20.
AguzziFBergamiMRGasparroCOccurrence of monoclonal components in general practice: Clinical implications. Eur J Haematol1992; 48: 192–5.
21.
PutnamFW. Alpha, beta, gamma, omega—the roster of the plasma proteins. In: PutnamFW ed. The Plasma Proteins. New York: Academic Press, 1975: 72–3.
