We describe a fast, simple and sensitive microliter scale competitive ELISA for the determination of urinary albumin. All reagents are commercially available. In the standard procedure, a minimum concentration of 0·8 mg albumin/L can be measured, although, applying another dilution, 0·04 mg/L can be detected. Within-batch coefficient of variation was 6·9% and 5·6% (at 1:20 and 1:50 dilution, respectively); between-batch variation was 8·6% and 5·6% respectively.
The influence of urine pH and other urine components is minimised in the assay diluting with a casein-containing buffer.
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