Free accessResearch articleFirst published online 1989-3
Simultaneous Assay for Urinary 4-Hydroxy-3-Methoxymandelic Acid,5-Hydroxyindoleacetic Acid and Homovanillic Acid by Isocratic HPLC with Electrochemical Detection
A method is described for the simultaneous determination of urinary HMMA, 5-HIAA and HVA by HPLC with electrochemical detection. The method was evaluated over an 8-month period and has thus far identified 2 cases of phaeochromocytoma, 1 case of neuroblastoma and 2 of carcinoid tumour.
References
1.
BeardCMShepsSGKurlandLTCarneyJALieJT. Occurrence of phaeochromocytoma in Rochester, Minnesota, 1950 through 1979. Mayo Clin Proc1983; 58: 802–4.
2.
BergeTLinellF. Carcinoid tumours. Frequency in a defined population during a 12-year period. Acta Path Microbiol Scand.1976; 84: 322–30.
3.
HodgsonHJFMatonPN. Carcinoid and neuroendocrine tumours of the liver. Clin Gastroenterol1987; 1: 35–61.
4.
ModlinIMFarndonJRShepherdAPhaeochromocytomas in 72 patients: clinical and diagnostic features, treatment and long term results. Br J Surg.1979; 66: 456–65.
5.
WeinkoveCLomaxKSwiftD. UKEQAS for urinary catecholamines. Commun Lab Med.1986; 2: 86–7.
TippettPAWestRSMcEwanAJMiddletonJEAckeryDM. A comparison of dopamine and homovanillic acid excretion, as prognostic indicators in malignant phaeochromocytoma. Clin Chim Acta1987; 166: 123–33.
8.
FeldmanJMBlalockJAZernRTDeficiency of dopamine-B-hydroxylase. A new mechanism for normotensive phaeochromocytomas. Am J Clin Pathol.1979; 72: 175–85.
9.
MoskowitzMA. Diseases of the autonomic nervous system. Clin Endocrinol Metab1977; 6: 745–68.
10.
FeldmanJM. Increased dopamine production in patients with carcinoid tumours. Metabolism1985; 34: 255–60.
11.
PisanoJJCroutJRAbrahamD. Determination of 3-methoxy-4-hydroxymandelic acid in urine. Clin Chim Acta1962; 7: 285–91.
12.
ConnelianTPGodfreyJM. The routine determination of urinary 4-hydroxy-3-methoxy mandelic acid. Clin Chim Acta1964; 9: 410–12.
13.
BrashearRJZeitvogelCHJacksonGJA fluorescent polarization immunoassay for the determination of urinary 5-hydroxy-3-indoleacetic acid. Clin Chem.1986; 32: 1122–3.
DavidsonDFFitzpatrickJ. A simple, optimised and rapid assay for urinary free catecholamines by HPLC with electrochemical detection. Ann Clin BioChem.1985; 22: 297–303.
16.
DeaconACBartlettWA. Interference by acetic acid in urinary 5-hydroxyindoleacetic acid determination. Clin Chem.1982; 28: 250–1.
17.
MailmanRBKiltsCD. Analytical considerations for quantitative determination of serotonin and its metabolically related products in biological matrices. Clin Chem.1985; 31: 1849–54.
18.
GatesSCSweeleyCCKrivitWDeWittDBlaisdellBE. Automated metabolic profiling of organic acids in human urine II. Analysis of urine samples from healthy adults, sick children and children with neuroblastoma. Clin Chem.1978; 24: 1680–9.
19.
KrstulovicAM. Investigations of catecholamine metabolism using high performance liquid chromatography. J Chromatog1982; 229: 1 34.
20.
DavidsonDFWilliamsonJ, A BASIC computer program for the comparison of analytical methods by Deming's method with an accompanying graph plot. Lab Practice1982; 31: 992–4.
21.
KaroumFAnahCORuthvenCRJSandlerM. Further observations on the gas chromatographic measurement of urinary phenolic acid indolic metabolites. Clin Chim Acta1969; 24: 341–8.
22.
ShihabiZKScaroJ. Liquid-chromatographic assay of urinary 5-hydroxy-3-indoleacetic acid, with electrochemical detection. Clin Chem.1980; 26: 907–9.
23.
ChouPPJaynesPKKingBPHolmesCSBaileyJL. Determination of urinary 5-hydroxyindole-3-acetic acid (5-HIAA) by HPLC with electrochemical detection. Clin Chem.1986; 32: 1590.
24.
PalfreySM. Determination of 4-hydroxy-3-methoxymandelic acid in urine using HPLC with electrochemical detection. Ann Clin BioChem.1987; 24: 172–6.
25.
NeftelKAGautschiK. Atenolol and pindolol interfere with urinary 5-hydroxyindole-3-acetic acid. Int J Clin Pharm Ther Toxicol1986; 24: 58–9.
26.
KnightJARobertsonGWuJT. The chemical basis and specificity of the nitrosonaphthol reaction. Clin Chem.1983; 29: 1969–71.
27.
ReevesPRMcAinshJMcIntoshDADWinrowMJ. Metabolism of atenolol in man. Xenobiotica1978; 8: 313–20.
28.
DavidsonDF. Urinary catecholamine assay by HPLC: In vitro interference by some drugs. Ann Clin BioChem.1988; 25: 583–4.
29.
ParkerNCLevtzowCBWrightPWWoodardLLChapmanJF. Uniform chromatographic conditions for quantifying urinary catecholamines, metaneph-rines, vanillylmandelic acid, 5-hydroxy-indoleacetic acid, by liquid chromatography, with electrochemical detection. Clin Chem.1986; 32: 1473–6.
30.
DavidsonDF. Urinary free catecholamines — diagnostic application of an HPLC technique to the investigation of neural crest tumours. Ann Clin BioChem.1987; 24: 494–499.
31.
RodeJDhillonAPCottonPBWoolfAO'RiordanJLH. Carcinoid tumour of stomach and primary hyperparathyroidism: a new association. J Clin Pathol.1987; 40: 546–51.
