Abstract
Simultaneous localisation of the isoenzymes of alkaline phosphatase and γ-glutamyl transferase was carried out on 173 serum samples from patients with hepatobiliary and bone disease to determine (i) if a better discrimination of the underlying pathology could be obtained by such an approach, and (ii) if the pathology suggested by the alkaline phosphatase isoenzyme separation could help in determining the cellular location of the γ-glutamyl transferase isoenzymes.
It was found that the localisation of the γ-glutamyl transferase isoenzymes had, with a few exceptions, little advantage over the measurement of the total activity but that some evidence concerning the cellular location of the γ-glutamyl transferase isoenzymes could be gained by such an approach. There was support for the view that the α2-migrating isoenzyme is derived from the liver parenchymal cytoplasm, but little support for the view that the α1-migrating isoenzyme is predominantly the induced microsomal enzyme or that the β-migrating isoenzyme is the result of impaired billiary secretion.