Antisera, raised against 2′-O-succinyl-cGMP conjugated to ovalbumin or kehole limpet haemocyanin, have been used to develop radioimmunoassays for the direct determination of urine and plasma cGMP levels. Details of these assays are presented, together with evidence for their validity.
References
1.
BlochA. (1975a). Uridine 3′,5′-monophosphate (cyclic UMP). I. Isolation from rat liver extracts. Biochemical and Biophysical Research Communications, 64, 210–218
2.
BlochA. (1975b). Isolation of cytidine 3′,5′-monophosphate from mammalian tissues and body fluids and its effects on leukemia L-1210 cell growth in culture. Advances in Cyclic Nucleotide Research, 5, 331–338
3.
BrownB. L.AlbanoJ. D. M.EkinsR. P.SgherziA. M.TampionW. (1971). A simple and sensitive saturation assay method for the measurement of adenosine 3′:5′-cyclic monophosphate. Biochemical Journal, 121, 561–562
4.
EkinsR. P. (1974). Radioimmunoassay and saturation analysis: Basic principles and theory. British Medical Bulletin, 30, 3–11
5.
FallonA. M.WyattG. R. (1975). An improved assay for cyclic GMP using an insect binding protein. Annals of Biochemistry and Experimental Medicine, 63, 614–619
6.
GoldbergN. D.HaddoxM. K.NicolS. E.GlassD. B.SandfordC. H.KuehlF. A.JrEstensenR. (1975). Biologic regulation through opposing influences of cyclic GMP and cyclic AMP: The Yin Yang hypothesis. Advances in Cyclic Nucleotide Research, 5, 307–330
7.
KobayashiR.FangV. S. (1975). A simple and sensitive competitive protein-binding assay for cyclic GMP. Biochemical and Biophysical Research Communications, 67, 493–500
8.
LebeauM.DumontJ. E.GolsteinJ. (1975). Urinary cyclic AMP and cyclic GMP during the menstrual cycle. Hormone and Metabolic Research, 7, 190–194
9.
MarksV.MorrisB. A.TealeJ. D. (1974). Radioimmunoassay and saturation analysis: Pharmacology. British Medical Bulletin, 30, 80–86
10.
MuradF.ManganielloV.VaughanM. (1971). A simple, sensitive protein-binding assay for guanosine 3′:5′-monophosphate. Proceedings of the National Academy of Sciences of the United States of America, 68, 736–739
11.
MuradF.PakC. Y. (1972). Urinary excretion of adenosine 3′,5′-monophosphate and guanosine 3′,5′ monophosphate. New England Journal of Medicine, 286, 1382–1287
12.
Nature (1973). News and Views: From cyclic AMP to cyclic GMP. Nature, 246, 186–187
13.
NeethlingA. C.ShanleyB. C. (1976). Letter: Cyclic GMP excretion and hepatoma. Lancet, 2, 578
14.
RosmanP. M.AgrawalR.GoodmanA. D.SteinerA. L. (1976). Effect of angiotensin II on cyclic guanosine monophosphate and cyclic adenosine monophosphate in human plasma. Journal of Clinical Endocrinology and Metabolism, 42, 531–536
15.
RudmanD.FleischerA.KutnerM. H. (1976). Concentration of 3′, 5′ cyclic adenosine monophosphate in ventricular cerebrospinal fluid of patients with prolonged coma after head trauma or intracranial hemorrhage. New England Journal of Medicine, 295, 635–638
16.
SiddleK.DaviesC. J.ShettyK. J.ElkelesR. S. (1976). The effect of insulin on adenosine 3′:5′-monophosphate and guanosine 3′:5′-monophosphate concentrations in human plasma. Clinical Science and Molecular Medicine, 50, 487–491
17.
SteinerA. L.PagliaraA. S.ChaseL. R.KipnisD. M. (1972a). Radioimmunoassay for cyclic nucleotides. II. Adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate in mammalian tissues and body fluids. Journal of Biological Chemistry, 247, 1114–1120
18.
SteinerA. L.ParkerC. W.KipnisD. M. (1970). The measurement of cyclic nucleotides by radioimmunoassay. Advances in Biochemical Psychopharmacology, 3, 89–111
19.
SteinerA. L.WehmannR. E.ParkerC. W.KipnisD. M. (1972b). Radioimmunoassay for the measurement of cyclic nucleotides. Advances in Cyclic Nucleotide Research, 2, 51–61
20.
ToveyK. C.OldhamK. G.WhelanJ. A. M. (1974). A simple direct assay for cyclic AMP in plasma and other biological samples using an improved competitive protein binding technique. Clinica chimica acta, 56, 221–234
21.
WoodP.EnglishJ.ChakrabortyJ.HintonR. (1975). The use of industrial non-ionic detergents in liquid scintillation counting. Laboratory Practice, 24, 739–740
22.
WoodP.HartmanG.MarksV. (1976). A simple and specific locust protein binding assay for cyclic 3′,5′ GMP. Biochemical and Biophysical Research Communications, 71, 1139–1146
