Surface-Enhanced Raman Spectroscopy Detection for Fenthion Pesticides Based on Gold Molecularly Imprinted Polymer Solid-State Substrates

Abstract

Current label-free surface-enhanced Raman spectroscopy (SERS) assay for the detection and analysis of organophosphorus pesticides has achieved initial success, but the application still faces constraints of substrate portability and specificity. To this end, this paper demonstrates a method for portable, rapid, and specific detection of low concentrations of fenthion pesticides based on a solid substrate of gold nanoparticle monolayers combined with molecularly imprinted polymers (MIPs). The nano-monolayers were transferred to the surface of mercapto-silicon wafers by interfacial self-assembly technique to form a stable connection with S–Au bonds and, at the same time, prevent nanoparticles from dropping off during the surfactant removal process. Then, the fenthion MIPs were directly generated on the surface of the monolayer film by spin-coating with a pre-polymerization solution and ultraviolet-induced polymerization. Tests showed that the molecular imprint was able to accurately bind to fenthion, but not other molecules, in a mixture of structural analogs, achieving a low concentration detection of 10^–8 mol/L. The composite substrate maintained a signal uniformity of a relative standard deviation (RSD)  =  7.05% and a batch-to-batch reproducibility of RSD  =  10.40%, making it a potential pathway for the extended application of SERS technology.