A series of 41 nasal polyps (23 single and 18 massive) and 6 normal nasal mucosa specimens was glycohistochemically investigated. Five plant lectins were used, ie, the peanut agglutinin (PNA), the wheat germ agglutinin (WGA), the gorse seed agglutinin (UEA-I), the Maackia amurensis agglutinin (MAA), and the elderberry bark agglutinin (SNA). A neoglycoconjugate and 2 animal lectins (CL-14 and CL-16) were also used. Three quantitative features were calculated by means of computer-assisted microscopy: the percentage of tissue area specifically stained by the histochemical probe, the staining intensity, and the heterogeneity level of the staining distribution. The results show that with respect to sialic acid-glycoprotein binding characteristics as determined by SNA, MAA, and WGA probes, the normal nasal mucosa differed markedly (p < .00001) from the polyposal one. The single nasal polyps exhibited glycohistochemical characteristics that differed markedly (p = .0004) from those exhibited by the massive ones. These differences related mainly to the UEA-I, PNA, and the Thomsen-Friedenreich antigen—exposing neoglycoprotein binding characteristics. In conclusion, the present study shows unambiguously that polyposal mucosa, whether of the single or the massive type, exhibits markedly distinct glycohistochemical characteristics when compared to normal nasal mucosa, and that single nasal polyps also differ markedly from massive ones.
JankowskiRBeneMCMonoret-VautrinAD. Immunohistological characteristics of nasal polyps. Rhinology1989; 8: 51–8.
2.
BernsteinJGorfienJNobleB. Role of allergy in nasal polyposis: A review. Otolaryngol Head Neck Surg1995; 113: 724–32.
3.
ShatkinJSDelsupeheKGThistedRACoreyJP. Mucosal allergy in the absence of systemic allergy in nasal polyposis and rhinitis: A meta analysis. Otolaryngol Head Neck Surg1994; 111: 553–6.
4.
HellquistHB. Pathology of the nose and paranasal sinuses. London, England: Butterworths, 1990: 24–32.
5.
HyamsVJBatsakisJGMichaelsL. Nasal and paranasal polyposis. In: HyamsVJBatsakisJGMichaelsL, eds. Tumors of the upper respiratory tract and ear. Fascicle 25. Second series. Washington, DC: Armed Forces Institute of Pathology, 1988: 11.
HassidSDecaesteckerCHermansC. Algorithm analysis of lectin glycohistochemistry and Feulgen cytometry for a new classification of nasal polyposis. Ann Otol Rhinol Laryngol1997; 106: 1043–51.
8.
GabiusHJWosgienBBrinckUSchauerA. Localization of endogenous β-galactoside—specific lectins by neoglycoproteins, lectin-binding tissue glycoproteins and antibodies and of accessible lectin-specific ligands by mammalian lectin in human breast carcinomas. Pathol Res Pract1991; 187: 839–47.
9.
HassidSSalmonIBrugmansM. Histochemical study of epithelia of nasal polyps by biotinylated lectins and neoglycoproteins. A comparison with the normal respiratory epithelium. Eur J Morphol1997; 35: 77–85.
SchnellerMAndreSCihakJ. Differential binding of two chicken β-galactoside—specific lectins to homologous lymphocyte subpopulations and evidence for inhibitor activity of the dimeric lectin on stimulated T cells. Cell Immunol1995; 166: 35–43.
12.
PerilloNLPaceKESeilhamerJJBaumLG. Apoptosis of T cells mediated by galectin-1. Nature1995; 378: 736–9.
13.
YamaokaAKuwaharaIFrigeriLGLiuFT. A human lectin, galectin-3 (εbp/Mac-2), stimulates superoxide production by neutrophils. J Immunol1995; 154: 3479–87.
14.
YangRYHsuDKLiuFT. Expression of galectin-3 modulates T-cell growth and apoptosis. Proc Natl Acad Sci USA1996; 93: 6737–42.
SolisDRomeroAKaltnerHGabiusHJDiaz-MaurinoT. Different architecture of the combining site of the two chicken galectins revealed by chemical mapping studies with synthetic ligand derivatives. J Biol Chem1996; 271: 12744–8.
17.
DanguyAKayserKBovinNVGabiusHJ. The relevance of neoglycoconjugates for histology and pathology. Trends Glycosci Glycotechnol1995; 7: 261–75.
18.
GoldschmidtDDecaesteckerCBertheJV. The contribution of computer-assisted methods for histopathological classification of adipose tumors. Lab Invest1996; 75: 295–306.
GabiusHJGabiusS, eds. Glycosciences: status and perspectives. London, England: Chapman and Hall, 1997.
23.
HakomoriS. Aberrant glycosylation in tumors and tumor-associated carbohydrate antigens. Adv Cancer Res1989; 52: 257–331.
24.
DabelsteenE. Cell surface carbohydrates as prognostic markers in human carcinomas. J Pathol1996; 179: 358–69.
25.
JannotMCKruczynskiALimouzyA. Spontaneous evolution of cytoplasmic lectin binding and nuclear size and deoxyribonucleic acid content in human colorectal cancers grafted onto nude mice. Lab Invest1993; 68: 446–55.
26.
KayserKBovinNVKorchaginaEYZeilingerCZengFYGabiusHJ. Correlation of expression of binding sites for the synthetic blood group A-, B-, and H-trisaccharides and for sarcolectin with survival of patients with bronchial carcinoma. Eur J Cancer1994; 30A: 653–7.
27.
XuXCEl-NaggarAKLotanR. Differential expression of galectin-1 and galectin-3 in thyroid tumors. Potential diagnostic implications. Am J Pathol1995; 147: 815–22.
28.
SalmonICambyIRemmelinkM. Lectin histochemistry, ploidy level and proliferation indices in meningioma subtypes. Neuropathol Appl Neurobiol1996; 22: 68–76.
29.
RothJ. Cellular sialoglycoconjugates: A histochemical perspective. Histochem J1993; 25: 687–710.
VarkiA. Sialic acids as ligands in recognition phenomena. FASEB J1997; 11: 248–55.
32.
PaulsonJCColleyKJ. Glycosyltransferases. Structure, localization and control of cell-type specific glycosylation. J Biol Chem1989; 264: 17615–8.
33.
ShibuyaNGoldsteinIJBroekaertWFNsimba-LubakiMPeetersBPeumansWJ. The elderberry (Sambucus nigra) bark lectin recognizes the Neu5Ac(α2,6)Gal/GalNAc sequence. J Biol Chem1987; 262: 1596–601.
34.
WangWCCummingsRD. The immobilized leukoagglutinin from the seeds of Maackia amurensis binds with high affinity to complex-type Asn-linked oligosaccharides containing terminal sialic acid-linked α2,3 to penultimate galactose residues. J Biol Chem1988; 263: 4576–85.
35.
DebrayHDecoutDStreckerGSpikGMontreuilJ. Specificity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycoproteins. Eur J Biochem1981; 117: 41–55.
36.
BarondesSHCastronovoVCooperDNW. Galectins: A family of animal β-galactoside—binding lectins. Cell1994; 76: 597–8.
37.
OhannesianDWLotanR. Galectins in tumor cells. In: GabiusHJGabiusS, eds. Glycosciences: Status and perspectives. London, England: Chapman and Hall, 1997: 459–69.
38.
KasaiKHirabayashiJ. Galectins: A family of animal lectins that decipher glycocodes. J Biochem (Tokyo)1996; 119: 1–8.
KayserKKaltnerHDongX. Prognostic relevance of detection of ligands for vertebrate galectins and a Lewisy-specific monoclonal antibody: A prospective study of bronchial carcinoma patients treated surgically. Int J Oncol1996; 9: 893–900.
