The in vivo effect of systemic administration of monoclonal antibody (mAb) against ICAM-1 (αICAM-1) in experimental immune-mediated labyrinthitis was evaluated. The αICAM-1–treated rats showed reduced inflammatory cell infiltration in the scala tympani and the perisaccular tissue of the endolymphatic sac. However, with this experimental paradigm, labyrinthitis could not be completely abolished. These findings suggest that ICAM-1–dependent pathways play an important role in the series of immunologic events occurring in the inner ear, and that the use of ICAM-1 antagonist may be a possible therapeutic approach to labyrinthitis.
TakahashiMHarrisJP. Analysis of immunocompetent cells following inner ear immunostimulation. Laryngoscope1988; 98: 1133–8.
2.
PoberJSCotranRS. The role of endothelial cells in inflammation. Transplantation1990; 50: 537–44.
3.
StearnsGSKeithleyEMHarrisJP. Development of high endothelial venule-like characteristics in the spiral modiolar vein induced by viral labyrinthitis. Laryngoscope1993; 103: 890–8.
4.
SuzukiMHarrisJP. Expression of intercellular adhesion molecule-1 during inner ear inflammation. Ann Otol Rhinol Laryngol1995; 104: 69–75.
5.
DoughertyGJMurdochSHoggN. The function of human intercellular adhesion molecule-1 (ICAM-1) in the generation of an immune response. Eur J Immunol1988; 18: 35–9.
6.
CosimiABContiDDelmonicoFL. In vivo effects of monoclonal antibody to ICAM-1 (CD54) in nonhuman primates with renal allografts. J Immunol1990; 144: 4604–12.
7.
CharltonBGuymerRHSlatteryRMMandelTE. Intercellular adhesion molecule (ICAM-1) inhibition can induce tolerance in vivo. Immunol Cell Biol1991; 69: 89–93.
8.
NishikawaKGuoYJMiyasakaM. Antibodies to intercellular adhesion molecule 1/lymphocyte function-associated antigen 1 prevent crescent formation in rat autoimmune glomerulonephritis. J Exp Med1993; 177: 667–77.
9.
BevilacquaMPNelsonRMMannoriGCecconiO. Endothelial-leukocyte adhesion molecules in human disease. Annu Rev Med1994; 45: 361–78.
10.
HarrisJP. Immunology of the inner ear: response of the inner ear to antigen challenge. Otolaryngol Head Neck Surg1983; 91: 18–23.
11.
HarrisJP. Immunology of the inner ear: evidence of local antibody production. Ann Otol Rhinol Laryngol1984; 93: 157–62.
12.
TamataniTMiyasakaM. Identification of monoclonal antibodies reactive with the rat homolog of IC AM-1, and evidence for a differential involvement of ICAM-1 in the adherence of resting versus activated lymphocytes to high endothelial cells. Int Immunol1990; 2: 165–71.
13.
DustinMLRothleinRBhanAKDinarelloCASpringerTA. Induction by IL 1 and interferon-g: tissue distribution, biochemistry, and function of anatural adherence molecule (IC AM-1). J Immunol1986; 137: 245–54.
14.
GloddekBHarrisJP. Role of lymphokines in the immune response of the inner ear. Acta Otolaryngol (Stockh) 1989; 108: 68–75.
15.
RothleinRMainolfiEAKishimotoTK. Treatment of inflammation with anti-ICAM-1. Res Immunol1993; 144: 735–9.
16.
LipskyPERosenthalAS. Macrophage-lymphocyte interaction: II. Antigen-mediated physical interactions between immune guinea pig lymph node lymphocytes and syngeneic macrophages. J Exp Med1975; 141: 138–54.
17.
SpringerTA. Adhesion receptors of the immune system. Nature1990; 346: 425–34.
DarmstadtGLKeithleyEMHarrisJP. Effects of cyclophosphamide on the pathogenesis of cytomegalovirus-induced labyrinthitis. Ann Otol Rhinol Laryngol1990; 99: 960–8.
20.
HaugCEColvinRBDelmonicoFL. A phase I trial of immunosuppression with anti—ICAM-1 (CD54) mAb in renal allograft recipients. Transplantation1993; 55: 766–73.
21.
KavanaughAFDavisLSNicholsLA. Treatment of refractory rheumatoid arthritis with a monoclonal antibody to intercellular adhesion molecule 1. Arthritis Rheum1994; 37: 992–9.
