Keratins as Molecular Markers of Epithelial Differentiation: Differential Expression in Crypt Epithelium of Human Palatine Tonsils

Abstract

The expression of keratins in the stratified squamous nonkeratinizing epithelium lining the surface and the crypts of human palatine tonsils was analyzed by high-resolution gel electrophoresis, immunoblotting, and immunohistochemical techniques. In contrast to the superficial epithelium, which showed a fairly constant keratin composition consisting of the neutral-to-basic keratins K4, K5, K6, and K8 and the acidic keratins K13, K14, K16, and K19, the keratin profiles of tonsillar crypt epithelial cells were found to be more variable, particularly with respect to the expression levels of K4 and K13. These were identical to those of surface epithelium, reduced, or abolished. Since K4 and K13 characterize the mature stage of differentiation in squamous nonkeratinizing epithelia, their decrease is indicative of an incomplete epithelial differentiation. Immunohistochemical analyses confirmed this hypothesis and allowed us, furthermore, to correlate the expression of K13 with the morphologic alterations of tonsillar crypt epithelium in the course of reticulation.

Keywords

biochemical and immunohistochemical analysis epithelial differentiation keratin expression palatine tonsil tonsillar crypt epithelium

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References

Fioretti

. Die Gaumenmandel. Stuttgart, Federal Republic of Germany: G Thieme, 1961.

Falk

Mootz

. Morphologische Untersuchungen zur Retikulierung des Tonsillenepithels. Acta Otolaryngol (Stockh) 1973; 75: 85–103.

Brandtzaeg

. Immune functions of human nasal mucosa and tonsils in health and disease. In: Bienenstock

, ed. Immunology of the lungs and upper respiratory tract. New York, NY: McGraw-Hill, 1984: 28–96.

Kawabata

. The surface structure of human tonsillar crypt. Scanning electron microscopic observation. Jpn J Tonsil 1978; 17: 204–10.

Howie

. Scanning and transmission electron microscopy on the epithelium of human palatine tonsils. J Pathol 1980; 130: 91–8.

Quinlan

Schiller

Hatzfeld

. Patterns of expression and organization of cytokeratin intermediate filaments. Ann NY Acad Sci 1985; 455: 126–43.

Cooper

Schermer

Sun

. Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: Strategies, applications, and limitations. Lab Invest 1985; 52: 243–56.

Osborn

. Summary: Intermediate filaments 1984. Ann NY Acad Sci 1985; 455: 669–81.

Moll

Franke

Schiller

Geiger

Krepler

. The catalogue of human cytokeratin polypeptides: Patterns of expression of specific cytokeratins in normal epithelia, tumors, and cultured cells. Cell 1982; 31: 11–24.

10.

Heid

Werner

Franke

. The complement of native α-keratin polypeptides of hair-forming cells: A subset of eight polypeptides that differ from epithelial cytokeratins. Differentiation 1986; 32: 101–19.

11.

Eichner

Sun

Aebi

. The role of keratin subfamilies and keratin pairs in the formation of human epidermal intermediate filaments. J Cell Biol 1986; 102: 1767–77.

12.

Hatzfeld

Franke

. Pair formation and promiscuity of cytokeratins: Formation in vitro of heterotypic complexes and intermediate-sized filaments by homologous and heterologous recombinations of purified polypeptides. J Cell Biol 1985; 101: 1826–41.

13.

Milstone

McGuire

. Different polypeptides form the intermediate filaments in bovine hoof and esophageal epithelium and in aortic endothelium. J Cell Biol 1981; 88: 312–6.

14.

Wild

Mischke

. Variation and frequency of cytokeratin polypeptide patterns in human squamous non-keratinizing epithelium. Exp Cell Res 1986; 162: 114–26.

15.

Mischke

Wild

. Polymorphic keratins in human epidermis. J Invest Dermatol 1987; 88: 191–7.

16.

Wild

Wille

Mischke

. Lateral cervical (branchial) cyst epithelia express upper digestive tract-type cytokeratins. Polyclonal antibody studies. Ann Otol Rhinol Laryngol 1988; 97: 365–72.

17.

Viac

Staquet

Thivolet

Goujon

. Experimental production of antibodies against stratum corneum keratin polypeptides. Arch Dermatol 1980; 267: 179–88.

18.

Osborn

Debus

Weber

. Monoclonal antibodies specific for vimentin. Eur J Cell Biol 1984; 34: 137–43.

19.

Cordell

Falini

Erber

. Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 1984; 32: 219–29.

20.

Ochs

. Protein contaminants of sodium dodecyl sulfatepolyacrylamide gels. Anal Biochem 1983; 135: 470–4.

21.

Ouhayoun

Gosselin

Forest

Winter

Franke

. Cytokeratin patterns of human oral epithelia: Differences in cytokeratin synthesis in gingival epithelium and the adjacent alveolar mucosa. Differentiation 1985; 30: 123–9.

22.

Geisler

Weber

. Amino acid sequence data on glial fibrillary acidic protein (GFAP): Implications for the subdivision of intermediate filaments into epithelial and nonepithelial members. EMBO J 1983; 2: 2059–63.