Collagenous components were isolated chemically from fetal bovine or guinea pig cochlear bone and human stapes after stapedectomy, and the purified protein was characterized by immunoblot assay and amino acid analysis. The results of this study suggest that these are mixtures of type I and type II collagens. The presence of type II collagen in the human stapes also was demonstrated by immunohistologic methods using monoclonal antibody. The presence of type II collagen in these tissues is significant, since it has been postulated as an autoantigen in autoimmune inner ear disease.
VosteenK-HSchuknechtHPfaltzCR, eds. Meniere's disease: Pathogenesis, diagnosis and treatment. New York: Georg Thieme Verlag, 1981
4.
SchuknechtHF. Meniere's disease, pathogenesis and pathology. Am J Otolaryngol1982; 3: 349–52
5.
YooTJTomodaKStuartJMKangAHTownesAS. Type II collagen-induced autoimmune otospongiosis. A preliminary report. Ann Otol Rhinol Laryngol1983; 92: 103–8
6.
YooTJ. Etiopathogenesis of otosclerosis: A hypothesis. Ann Otol Rhinol Laryngol1984; 93: 28–33
7.
YooTJTomodaKStuartJMCremerMATownesASKangAH. Type II collagen-induced autoimmune sensorineural hearing loss and vestibular dysfunction in rats. Ann Otol Rhinol Laryngol1983; 92: 267–71
8.
YooTJTomodaKHernandezAD. Type II collagen-induced autoimmune inner ear lesions in guinea pigs. Ann Otol Rhinol Laryngol1984; 93(suppl 113): 3–5
9.
YooTJStuartJMKangAHTownesASTomodaKDixitS. Type II collagen autoimmunity in otosclerosis and Meniere's disease. Science1982; 217: 1153–5
10.
LammeliUK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature1970; 227: 680–2
11.
VollerABirdwellEBartlettA. The application of microplate enzyme-linked immunoabsorbent assays to some infectious diseases. In: FeldmanC, ed. Immunoenzymatic technique. Amsterdam: North-Holland, 1976: 167–73
12.
AvnameasSTandouBTernynekT. Specificity of antibodies synthesized by immunolocytes as detected by immunoenzyme techniques. Int Arch Allergy Appl Immunol1971; 40: 161–70
13.
KohlerGMilsteinC. Continuous cultures of fused cells secreting antibody of pre-defined specificity. Nature1975; 256: 495–7
14.
EpsteinEHScottRDMillerEJPiezKA. Isolation and characterization of the peptides derived from soluble human and baboon skin collagen after cyanogen bromide cleavage. J Biol Chem1971; 246: 1718–24
15.
MillerEJvan der KorstJKSokaloffL. Collagen of human articular and costal cartilage. Arthritis Rheum1969; 12: 21–9
16.
TimplRvon der MarkKvon der MarkH. Immunochemistry and immunohistology of collagens. In: ViidikAVuustJ, eds. Biology of collagen. New York: Academic Press, 1980: 211–22
17.
MillerEJ. The structure of fibril-forming collagens. Ann NY Acad Sci1985; 406: 1–13
18.
BirchallJPPearmanKDawesJDSykesB. The collagens of middle-ear structures and tympanosclerotic plaques. J Laryngol Otol1982; 96: 797–800
19.
YooTJTomodaK. Type II collagen distribution in the external and middle ear. Read before the Sixth Midwinter Meeting of the Association for Research in Otolaryngology, St Petersburg, Fla, February 1983: 111
