To elucidate the possible role of papillomaviruses as etiological agents in nasal inverting papillomas, DNA hybridization techniques were used. Total DNA from two nasal inverting papillomas was examined for the presence of DNA from human papillomavirus (HPV) types 6 and 11 under stringent conditions of hybridization. Both lesions contained DNA hybridizing with HPV 11. Restriction enzyme digestion and subsequent Southern blotting of the DNA samples revealed that one lesion contained viral DNA identical to HPV 11a. The DNA of the other lesion contained an extra 500 base pair insertion. These results provide definitive evidence for the first time for the association of HPV with nasal inverting papillomas.
RingertzN. Tumors of the nose and paranasal sinuses. Acta Otolaryngol (Stockh) [Suppl]1938; 27: 31–42
2.
MajumdarBBeckS. Inverted papilloma of the nose. Some aspects of aetiology. J Laryngol Otol1984; 98: 467–70
3.
BatsakisJG. Nasal (schneiderian) papillomas. Ann Otol Rhinol Laryngol1981; 90: 190–1
4.
RidolfiRLLiebermanPHErlandsonRAMooreOS. Schneiderian papillomas: A clinico-pathologic study of 30 cases. Am J Surg Pathol1977; 1: 43–53
5.
HyamsVJ. Papillomas of the nasal cavity and paranasal sinuses. Ann Otol Rhinol Laryngol1971; 80: 192–206
6.
SuhKWFacerGWDevineKDWeilandLHZujkoRD. Inverting papilloma of the nose and paranasal sinuses. Laryngoscope1977; 87: 35–46
7.
VrabecDP. The inverted schneiderian papilloma: A clinical and pathological study. Laryngoscope1975; 85: 186–220
8.
SkolnikEMLoewyAFriedmanJE. Inverted papilloma of the nasal cavity. Arch Otolaryngol1966; 84: 61–7
9.
BatsakisJG. Tumors of the head and neck: Clinical and pathological considerations. 2nd ed.Baltimore: Williams and Wilkins, 1979: 130–7
10.
EaveyRD. Inverted papillomas of the nose and paranasal sinuses in childhood and adolescence. Laryngoscope1985; 95: 17–22
11.
KusiakRJHudsonWR. Nasal papillomatosis. South Med J1970; 63: 1277–80
12.
SnyderRNPerzinKH. Papillomatosis of nasal cavity and paranasal sinuses (inverted papilloma, squamous papilloma). Cancer1972; 30: 668–90
13.
CostaJHowleyPMBowlingMCHowardRBauerWC. Presence of human papilloma viral antigens in juvenile multiple laryngeal papilloma. Am J Clin Pathol1981; 75: 194–7
14.
StraussMJensonAB. Human papillomavirus in various lesions of the head and neck. Otolaryngol Head Neck Surg1985; 93: 342–6
15.
GaitoRAGaylordWHHildingDA. Ultrastructure of a human nasal papilloma. Laryngoscope1965; 75: 144–52
16.
SyrjanenKJPyrhonenSSyrjanenSt M. Evidence suggesting human papillomavirus (HPV) etiology for the squamous cell papilloma of the paranasal sinus. Arch Geschwulstforsch1983; 53: 77–82
17.
PaterMMPaterAFioriMSlotaJdiMayorcaG. BK virus DNA sequences in human tumor and normal tissues and cell lines. Vol 7. Viruses in naturally occurring cancers. Cold Spring Harbor Conf Cell Prolif1980; 7: 329–41
PaterMMPaterAdiMayorcaGBethEGiraldoG. BK virus transformed inbred hamster brain cells: Status of viral DNA in subclones. Mol Cell Biol1982; 2: 837–44
20.
PaterMMPaterA. Thymidine kinase of herpes virus as a vehicle for the isolation and characterization of unknown mammalian promoters and enhancers. J Mol Appl Genet1984; 2: 363–71
21.
GissmannLWolnikLIkenbergHKoldovskyUSchnurchHGzur HausenH. Human papillomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical cancers. Proc Natl Acad Sci USA1983; 80: 560–3
22.
GissmannLDiehlVSchultz-CoulonHzur HausenH. Molecular cloning and characterization of human papilloma virus DNA derived from a laryngeal papilloma. J Virol1982; 44: 393–400
23.
QuickCAFarasAKrzyzekR. The etiology of laryngeal papillomatosis. Laryngoscope1978; 88: 1789–95
24.
SchwartzEDurstMDemankowskiC. DNA sequence and genome organization of genital human papillomavirus type 6b. EMBO J1983; 2: 2341–8
