The influence of mild heat pretreatment on the bactericidal action of nisin, carvacrol and thymol on stationary and exponential phase cells of two Bacillus cereus strains was studied. Carvacrol or thymol concentrations of 0.3 mmol/L had no bactericidal effect on unheated, and a minor effect on heated B. cereus cells either in stationary or exponential growth phases. Nisin (0.15 mg/mL) alone decreased the viable counts in all cases (unheated and heated cells in stationary and exponential phase) although the two strains tested showed different sensitivity to this natural antimicrobial between them. When carvacrol or thymol were combined with nisin, it resulted in a greater loss of viability of heated cells than when nisin was applied alone. There was a synergistic effect of nisin and both essential oils with a mild heat pretreatment on the viability of B. cereus cells. This study pointed out the potential use of nisin alone and in combination with carvacrol or thymol applied after a mild heat pretreatment for preservation of minimally processed foods.
Beuchat L.R. (1994). Antimicrobial properties of spices and their essential oils. In: Dillon V.M. and Board R.G. (eds.), Natural Antimicrobial Systems and Food Preservation. Wallingford: Cab International. pp. 167–179.
2.
Beuchat L.R. , Rocelle M. , Clavero S. and Jaquette C.B. (1997). Effects of nisin and temperature on survival, growth, and enterotoxin production characteristics of psychrotrophic Bacillus cereus in beef gravy . Applied and Environmental Microbiology63(5): 1953–1958.
3.
Carlin F. , Guinebretiere M.H. , Choma C. , Pasqualini R. , Braconnier A. and Nguyen-the C. (2000). Spore-forming bacteria in commercial cooked, pasteurised and chilled vegetable purees . Food Microbiology17(2): 153–165.
4.
Delves-Broughton J. and Gasson M.J. (1994). Nisin. In: Dillon V.M. and Board R.G. (eds.), Natural Antimicrobial Systems and Food Preservation. Wallingford: Cab International. pp. 99–131.
5.
Delves-Broughton J. , Blackburn P. , Evans R.J. and Hugenholtz J. (1996). Applications of the bacteriocin, nisin . Antonie van Leeuwenhoek International Journal General and Molecular Microbiology69(2): 193–202.
6.
Dillon V.M. and Board R.G. (1994). Future prospects for natural antimicrobial food preservation systems. In: Dillon, V.M. and Board, R.G. (eds.), Natural Antimicrobial Systems and Food Preservation. Oxon, UK: Cab International. pp. 297–305.
7.
Dufrenne J. , Soentoro P. , Tatini S. , Day T. and Notermans S. (1994). Characteristics of Bacillus cereus related to safe food production . International Journal of Food Microbiology23: 99–109.
8.
Ettayebi K. , El-Yamani J. and Rossi-Hassani B.-D. (2000). Synergistic effects of nisin and thymol on antimicrobial activities in Listeria monocytogenes and Bacillus subtilis. FEMS Microbiology Letters183: 191–195.
9.
Evans J.D. and Martin S.A. (2000). Effects of thymol on ruminal microorganisms . Current Microbiology41: 336–340.
10.
Gould G.W. (1996). Industry perspectives on the use of natural antimicrobials and inhibitors for food applications . Journal of Food Protectionsupplement: 82–86.
11.
Granum P.E. (1997). Bacillus cereus. In: Doyle M.P. , Beuchat L.R. and Montville T.J. (eds.), Food Microbiology: Fundaments and Frontiers. Massachusetts, Washington DC: American Society for Microbiology. pp.327–336.
12.
Granum P.E. and Lund T. (1997). Bacillus cereusand its food poisoning toxins . FEMS Microbiology Letters157: 223–228.
13.
Hassan G. and Nabbut N. (1996). Prevalence and characterization of Bacillus cereus isolates from clinical and natural sources . Journal of Food Protection59(2): 193–196.
14.
Helander I.M. , Alakomi H.-L. , Latva-Kala K. , Mattila-Sandholm T. , Pol I.E. , Smid E.J. , Gorris L.G.M. and von Wright A. (1998). Characterisation of the action of selected essential oil components on Gram-negative bacteria . Journal of Agriculture and Food Chemistry46: 3590–3595.
15.
Jack R.W. , Tagg J.R. and Ray B. (1995). Bacteriocins of Gram-positive bacteria . Microbiological Reviews59(2): 171–200.
16.
Juven B.J. , Kanner J. , Schved F. and Weisslowicz H. (1994). Factors that interact with the antibacterial action of thyme essential oil and its active constituent . Journal of Applied Bacteriology76: 626–631.
17.
Kim J. , Marshall M.R. and Wei C. (1995). Antibacterial activity of some essential oil components against five foodborne pathogens . Journal of Agricultural Food Chemistry43(11): 2839–2845.
18.
Lagouri V. , Blekas G. , Tsimidou M. , Kokkini S. and Boskou D. (1993). Composition and antioxidant activity of essential oil from oregano plants grown wild in Greece . Zeitschrift fuür Lebensmittel Untersuchung und Forschung197: 20–23.
19.
Mockute D. and Bernotiene G. (1999). The main citralgeraniol and carvacrol chemotypes of the essential oil of Thymus pulegioides L. growing wild in Vilnius District (Lithuania) . Journal of Agriculture and Food Chemistry47: 3787–3790.
20.
Periago P.M. and Moezelaar R. (2001). Combined effect of nisin and carvacrol at different pH and temperature levels on the viability of different strains of Bacillus cereus . International Journal of Food Microbiology68: 141–148.
21.
Periago P.M. , Wouters J.A. and Abee T. (2001). Identification of heat induced proteins in Bacillus cereus ATCC14579 and Bacillus weihenstephanensis DSM11821. In: NIZO Dairy Conference on Food Microbes. Ede, Holanda: Elsevier Science.
22.
Pol I.E. and Smid E.J. (1999). Combined action of nisin and carvacrol on Bacillus cereus and Listeria monocytogenes . Letters in Applied Microbiology29(3): 166–170.
23.
Ray B. (1992). Nisin of Lactococcus lactis ssp. lactis as a food biopreservative. In: Ray B. and Daeschel M.A. (eds.), Food biopreservatives of microbial origin. Florida, US: Boca Raton. pp. 207–264.
24.
Sivropoulou A. , Papanikolaou E. , Nikolaou C. , Kokkini S. , Lanaras T. and Arsenakis M. (1996). Antimicrobial and cytotoxic activities of Origanum essential oils . Journal of Agriculture and Food Chemistry44(5): 1202–1205.
25.
Thompson D.P. (1996). Inhibition of growth of mycotoxigenic Fusarium species by butylated hydroxyanisole and/or carvacrol . Journal of Food Protection59: 412–415.
26.
Ueckert J.E. , ter Steeg P.F. and Coote P.J. (1998). Synergistic antibacterial action of heat in combination with nisin and magainin II amide . Journal of Applied Microbiology85: 487–494.
27.
Ultee A. , Gorris L.G.M. and Smid E.J. (1998). Bactericidal activity of carvacrol towards the foodborne pathogen Bacillus cereus . Journal of Applied Microbiology85: 211–218.
28.
Ultee A. , Slump R.A. , Steging G. and Smid E.J. (2000). Antimicrobial activity of carvacrol towards Bacillus cereus on rice . Journal of Food Protection63(5): 620–624.
