Restricted accessResearch articleFirst published online 2024-10
Dark Tea Wine Protects Against Metabolic Dysfunction-Associated Steatotic Liver Disease In Vivo Through Activating the Nrf2/HO-1 Antioxidant Signaling Pathway
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a complex and multifactorial disease. Dark tea exhibits great potential for various bioactivities for metabolic health. In this study, we aimed to evaluate therapeutic effects and the underlying mechanisms of dark tea wine (DTW) on MASLD with obesity. A rat model of MASLD was established by high-fat diet and administered with different doses of DTW as an intervention. The biomarkers of lipid metabolism and oxidative stress in rats were tested. The weight of organs and adipose tissues and the expressions of nuclear factor erythroid 2-like 2 (Nrf2) and heme oxygenase-1 (HO-1) were investigated based on the pathology and western blot analysis. We found that DTW enhanced antioxidant capacity via activating the Nrf2/HO-1 signaling pathway, further markedly triggering inhibition of weight gain, reduction of lipid dysfunction, and improvement of pathological characteristics to ameliorate MASLD induced by high-fat diet. These results suggest that DTW is a promising functional supplement for prevention and treatment of MASLD and obesity.
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References
1.
KhanA, RossHM, ParraNS, et al.Risk prevention and health promotion for non-alcoholic fatty liver diseases (MASLD). Livers, 2022; 2(4):264–282; doi: 10.3390/livers2040022
2.
WangH, ZhuYY, WangL, et al.Mangiferin ameliorates fatty liver via modulation of autophagy and inflammation in high-fat-diet induced mice. Biomed Pharmacother, 2017; 96:328–335; doi: 10.1016/j.biopha.2017.10.022
YounossiZM, StepanovaM, RafiqN, et al.Pathologic criteria for nonalcoholic steatohepatitis: Interprotocol agreement and ability to predict liver-related mortality. Hepatology, 2011; 53(6):1874–1882; doi: 10.1002/hep.24268
5.
ZhouF, ZhouJH, WangWX, et al.Unexpected rapid increase in the burden of MASLD in China from 2008 to 2018: A systematic review and meta-analysis. Hepatology, 2019; 70(4):1119–1133; doi: 10.1002/hep.30702
6.
YounossiZM, KoenigAB, AbdelatifD, et al.Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence, incidence, and outcomes. Hepatology, 2016; 64(1):73–84; doi: 10.1002/hep.28431
7.
RotmanY, SanyalAJ. Current and upcoming pharmacotherapy for nonalcoholic fatty liver disease. Gut, 2017; 66(1):180–190; doi: 10.1136/gutjnl-2016-312431
ChenJYS, ChuaD, LimCO, et al.Lessons on drug development: A literature review of challenges faced in nonalcoholic fatty liver disease (MASLD) clinical trials. Int J Mol Sci, 2023; 24(1):158; doi: 10.3390/ijms24010158
10.
ZhengMH, FouadY, ChanWK, et al.A new definition for metabolic dysfunction-associated fatty liver disease: An international expert consensus statement. J Hepatol, 2020; 73(1):202–209; doi: 10.1016/j.jhep.2020.03.039
11.
ChenZ, TianRF, SheZG, et al.Role of oxidative stress in the pathogenesis of nonalcoholic fatty liver disease. Free Radic Biol Med, 2020; 152:116–141; doi: 10.1016/j.freeradbiomed.2020.02.025
12.
Arroyave-OspinaJC, WuZM, GengY, et al.Role of oxidative stress in the pathogenesis of non-alcoholic fatty liver disease: Implications for prevention and therapy. Antioxidants (Basel), 2021; 10(2):174; doi: 10.3390/antiox10020174
13.
LiuW, BakerSS, BakerRD, et al.Antioxidant mechanisms in nonalcoholic fatty liver disease. Curr Drug Targets, 2015; 16(12):1301–1314; doi: 10.2174/1389450116666150427155342
14.
ZhangMH, LiJ, ZhuXY, et al.Physalin B ameliorates nonalcoholic steatohepatitis by stimulating autophagy and NRF2 activation mediated improvement in oxidative stress. Free Radic Biol Med, 2021; 164:1–12; doi: 10.1016/j.freeradbiomed.2020.12.020
15.
QiuMY, XiaoFQ, WangTN, et al.Protective effect of Hedansanqi Tiaozhi Tang against non-alcoholic fatty liver disease in vitro and in vivo through activating Nrf2/HO-1 antioxidant signaling pathway. Phytomedicine, 2020; 67:153140; doi: 10.1016/j.phymed.2019.153140
16.
LiuC, GuoY, SunL, et al.Six types of tea reduce high-fat-diet-induced fat accumulation in mice by increasing lipid metabolism and suppressing inflammation. Food Funct, 2019; 10(4):2061–2074; doi: 10.1039/c8fo02334d
17.
FuDH, RyanEP, HuangJN, et al.Fermented Camellia sinensis, Fu Zhuan tea, regulates hyperlipidemia and transcription factors involved in lipid catabolism. Food Res Int, 2011; 44(9):2999–3005; doi: 10.1016/j.foodres.2011.07.008
18.
MengXH, LiN, ZhuHT, et al.Plant resources, chemical constituents, and bioactivities of tea plants from the genus Camellia Section Thea. J Agric Food Chem, 2019; 67(19):5318–5349; doi: 10.1021/acs.jafc.8b05037
19.
PengYX, XiongZ, LiJ, et al.Water extract of the fungi from Fuzhuan brick tea improves the beneficial function on inhibiting fat deposition. Int J Food Sci Nutr, 2014; 65(5):610–614; doi: 10.3109/09637486.2014.898253
20.
WangJ, LiuW, ChenZ, et al.Physicochemical characterization of the oolong tea polysaccharides with high molecular weight and their synergistic effects in combination with polyphenols on hepatocellular carcinoma. Biomed Pharmacother, 2017; 90:160–170; doi: 10.1016/j.biopha.2017.03.059
21.
YuM, WangD, YangW, et al.Mechanisms of Nrf2/HO-1 pathway up-regulation induced by Pu-Erh black tea extract supplementation for quinocetone-treated sprague-dawley rats. J. Funct. Food, 2015; 14:767–778; doi: 10.1016/j.jff.2015.03.004
22.
DengH, LiuJ, XiaoY, et al.Possible mechanisms of dark tea in cancer prevention and management: A comprehensive review. Nutrients, 2023; 15(18):3903; doi: 10.3390/nu15183903
23.
ZhengQ, LiW, ZhangH, et al.Optimizing synchronous extraction and antioxidant activity evaluation of polyphenols and polysaccharides from Ya’an tibetan Tea (Camellia sinensis). Food Sci Nutr, 2020; 8(1):489–499; doi: 10.1002/fsn3.1331
24.
ZhuJ, ZhouH, ZhangJ, et al.Valorization of polysaccharides obtained from dark tea: Preparation, physicochemical, antioxidant, and hypoglycemic properties. Foods, 2021; 10(10):2276; doi: 10.3390/foods10102276
25.
YangX, HuangM, QinC, et al.Structural characterization and evaluation of the antioxidant activities of polysaccharides extracted from qingzhuan brick tea. Int J Biol Macromol, 2017; 101:768–775; doi: 10.1016/j.ijbiomac.2017.03.189
26.
WangD, ZhongY, LuoX, et al.Pu-Erh black tea supplementation decreases quinocetone-induced ROS generation and oxidative DNA damage in Balb/c Mice. Food Chem Toxicol, 2011; 49(2):477–484; doi: 10.1016/j.fct.2010.11.028
27.
ZhengXN, WangXW, LiLY, et al.Pu-Erh tea powder preventive effects on cisplatin-induced liver oxidative damage in Wistar rats. Asian Pac J Cancer Prev, 2014; 15(17):7389–7394; doi: 10.7314/apjcp.2014.15.17.7389
28.
CaoSY, LiBY, GanRY, et al.The in vivo antioxidant and hepatoprotective actions of selected Chinese teas. Foods, 2020; 9(3):262; doi: 10.3390/foods9030262
29.
ZhengJS, FuYQ, ChenQ, et al.Consumption of Chinese tea-flavor liquor improves circulating insulin levels without affecting hepatic lipid metabolism-related gene expression in Sprague-Dawley rats. ScientificWorldJournal, 2013; 2013:842343; doi: 10.1155/2013/842343
30.
ChenKH, LiPC, LinWH, et al.Depression by a green tea extract of alcohol-induced oxidative stress and lipogenesis in rat liver. Biosci Biotechnol Biochem, 2011; 75(9):1668–1676; doi: 10.1271/bbb.110163
31.
QinY, YuanZJ, YangFY, et al.Development of a new type of Anhua black tea and its application: Black tea wine. J Food Process Pres, 2021; 46:e15862; doi: 10.1111/jfpp.15862
32.
FanW, QianMC. “Characterization of aroma compounds of Chinese “Wuliangye” and “Jiannanchun” liquors by aroma extract dilution analysis. J Agric Food Chem, 2006; 54(7):2695–2704; doi: 10.1021/jf052635t
33.
KongYS, RenHY, LiuR, et al.Microbial and nonvolatile chemical diversities of Chinese dark teas are differed by latitude and pile fermentation. J Agric Food Chem, 2022; 70(18):5701–5714; doi: 10.1021/acs.jafc.2c01005
34.
ZhuMZ, LiN, ZhouF, et al.Microbial bioconversion of the chemical components in dark tea. Food Chem, 2020; 312:126043; doi: 10.1016/j.foodchem.2019.126043
35.
TengHH, ZhangY, JinCS, et al.Polysaccharides from steam-processed Polygonatum cyrtonema Hua enhance antioxidant activity through the activation of Nrf2/HO-1 signaling. J Sci Food Agri, 2023; 103(2):779–791; doi: 10.1002/jsfa.12189
36.
WuDL, WangTS, LiuHJ, et al.Study on the mechanism of Wuzi-Yanzong-Wan-medicated serum interfering with the mitochondrial permeability transition pore in the GC-2 cell induced by atractyloside. Chin J Nat Med, 2022; 20(4):282–289; doi: 10.1016/S1875-5364(22)60153-5
37.
KuceraO, CervinkovaZ. Experimental models of non-alcoholic fatty liver disease in rats. World J Gastroenterol, 2014; 20(26):8364–8376; doi: 10.3748/wjg.v20.i26.8364
38.
AmirinejadA, TotmajAS, MardaliF, et al.Administration of hydro-alcoholic extract of spinach improves oxidative stress and inflammation in high-fat diet-induced MASLD rats. BMC Complement Med Ther, 2021; 21(1):221–230; doi: 10.1186/s12906-021-03396-x
39.
ChenJH, ZhouH, JinHW, et al.Role of inflammatory factors in mediating of the lipids on nonalcoholic fatty liver disease: A two-step, Multivariable Mendelian Randomization Study. Nutrients, 2022; 14(20):4434; doi: 10.3390/nu14204434
40.
HouXY, GuanYP, TangY, et al.A correlation study of the relationships between nonalcoholic fatty liver disease and serum triglyceride concentration after an oral fat tolerance test. Lipids Health Dis, 2021; 20(1):54; doi: 10.1186/s12944-021-01483-z
41.
ZhuWT, LiangA, ShiP, et al.Higher serum uric acid to HDL-cholesterol ratio is associated with onset of non-alcoholic fatty liver disease in a non-obese Chinese population with normal blood lipid levels. BMC Gastroenterol, 2022; 22(1):196–206; doi: 10.1186/s12876-022-02263-4
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