Although the bactericidal activity of other Salvia spp. has been documented, research on Salvia hispanica seeds is sparse. This study examined the effects of S. hispanica seed extracts against drug-resistant Staphylococcus aureus reference strains and clinical isolates. Antibacterial effect was assessed using the minimal inhibitory concentration. Antibiofilm activity was evaluated using S. aureus ATCC 43300 and SAU-UIMY-31 strains, and antihemolytic effects were evaluated using S. aureus ATCC 29213 and SAU-UIMY-31. Biological effects and phytochemical profiles were analyzed through gas chromatography and mass spectrometry and chemometric tools. The nonpolar extract demonstrated antibiofilm activity with IC50 values of 93.9 ± 5.4 µg/mL and 146.2 ± 2.4 µg/mL against S. aureus ATCC 43300 and SAU-UIMY-31, respectively. The median polar extract showed an IC50 of 168.1 ± 13.9 µg/mL against S. aureus ATCC 43,300 and >250 µg/mL against SAU-UIMY-31. Antihemolysis IC50 values for both extracts were 48.5 ± 3.3 and 52.9 ± 3.1 µg/mL against S. aureus ATCC 29213 and 58.0 ± 1.4 and 87.9 ± 2.4 µg/mL against SAU-UIMY-31, respectively. Chemometric analyses suggested that fatty acid methyl esters are potential contributors to biological activity. These findings provide a foundation for future studies on the use of S. hispanica seeds in antibacterial therapies.
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