The innate immune response is an important host primary defense system against pathogens. γ-Oryzanol is one of the nutritionally important phytoceutical components in rice bran oil. The goal of this study was to investigate the effect of γ-oryzanol-rich extract from black rice bran (γORE) on the activation of the innate immune system. In this study, we show that γORE increased the expression of CD14 and Toll-like receptor 4 and enhanced the phagocytic activity of RAW264.7 macrophages. Furthermore, γORE and its active ingredient γ-oryzanol promoted the secretion of innate cytokines, interleukin-8, and CCL2, which facilitate phagocytosis by RAW264.7 cells. These findings suggest that γ-oryzanol in the γORE enhances innate immune responses.
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References
1.
MedzhitovR, JanewayCJr.: Innate immunity. N Engl J Med, 2000; 343:338–344.
FriedmanM: Rice brans, rice bran oils, and rice hulls: Composition, food and industrial uses, and bioactivities in humans, animals, and cells. J Agric Food Chem, 2013; 61:10626–10641.
11.
CiceroAF, GaddiA: Rice bran oil and gamma-oryzanol in the treatment of hyperlipoproteinaemias and other conditions. Phytother Res, 2001; 15:277–289.
12.
IslamMS, MurataT, FujisawaM, et al.: Anti-inflammatory effects of phytosteryl ferulates in colitis induced by dextran sulphate sodium in mice. Br J Pharmacol, 2008; 154:812–824.
13.
AkihisaT, YasukawaK, YamauraM, et al.: Triterpene alcohol and sterol ferulates from rice bran and their anti-inflammatory effects. J Agric Food Chem, 2000; 48:2313–2319.
14.
YasukawaK, AkihisaT, KimuraY, TamuraT, TakidoM: Inhibitory effect of cycloartenol ferulate, a component of rice bran, on tumor promotion in two-stage carcinogenesis in mouse skin. Biol Pharm Bull, 1998; 21:1072–1076.
15.
SonMJ, RicoCW, NamSH, KangMY: Effect of oryzanol and ferulic acid on the glucose metabolism of mice fed with a high-fat diet. J Food Sci, 2011; 76:H7–H10.
16.
XuZ, HuaN, GodberJS: Antioxidant activity of tocopherols, tocotrienols, and gamma-oryzanol components from rice bran against cholesterol oxidation accelerated by 2,2′-azobis(2-methylpropionamidine) dihydrochloride. J Agric Food Chem, 2001; 49:2077–2081.
17.
MinSW, RyuSN, KimDH: Anti-inflammatory effects of black rice, cyanidin-3-O-beta-D-glycoside, and its metabolites, cyanidin and protocatechuic acid. Int Immunopharmacol, 2010; 10:959–966.
18.
ChoiSP, KimSP, KangMY, NamSH, FriedmanM: Protective effects of black rice bran against chemically-induced inflammation of mouse skin. J Agric Food Chem, 2010; 58:10007–10015.
19.
LimtrakulP, YodkeereeS, PitchakarnP, PunfaW: Suppression of inflammatory responses by black rice extract in RAW 264.7 macrophage cells via downregulation of NF-kB and AP-1 signaling pathways. Asian Pac J Cancer Prev, 2015; 16:4277–4283.
20.
ScavarielloEM, ArellanoDB: [Gamma-oryzanol: An important component in rice brain oil]. Arch Latinoam Nutr, 1998; 48:7–12.
21.
ShinSY, KimCG, JungYJ, LimY, LeeYH: The UPR inducer DPP23 inhibits the metastatic potential of MDA-MB-231 human breast cancer cells by targeting the Akt-IKK-NF-kappaB-MMP-9 axis. Sci Rep, 2016; 6:34134.
22.
YeoJC, WallAA, StowJL, HamiltonNA: High-throughput quantification of early stages of phagocytosis. Biotechniques, 2013; 55:115–124.
23.
KhabarKS, Al-ZoghaibiF, Al-AhdalMN, et al.: The alpha chemokine, interleukin 8, inhibits the antiviral action of interferon alpha. J Exp Med, 1997; 186:1077–1085.
24.
LorenzE: TLR2 and TLR4 expression during bacterial infections. Curr Pharm Des, 2006; 12:4185–4193.
QidwaiT, KhanMY: Impact of genetic variations in C-C chemokine receptors and ligands on infectious diseases. Hum Immunol, 2016; 77:961–971.
27.
TurnerMD, NedjaiB, HurstT, PenningtonDJ: Cytokines and chemokines: At the crossroads of cell signalling and inflammatory disease. Biochim Biophys Acta, 2014; 1843:2563–2582.
28.
SakaiS, MurataT, TsubosakaY, UshioH, HoriM, OzakiH: gamma-Oryzanol reduces adhesion molecule expression in vascular endothelial cells via suppression of nuclear factor-kappaB activation. J Agric Food Chem, 2012; 60:3367–3372.
29.
GayNJ, SymmonsMF, GangloffM, BryantCE: Assembly and localization of Toll-like receptor signalling complexes. Nat Rev Immunol, 2014; 14:546–558.
30.
XuLL, WarrenMK, RoseWL, GongW, WangJM: Human recombinant monocyte chemotactic protein and other C-C chemokines bind and induce directional migration of dendritic cells in vitro. J Leukoc Biol, 1996; 60:365–371.
31.
GomesRN, Teixeira-CunhaMG, FigueiredoRT, et al.: Bacterial clearance in septic mice is modulated by MCP-1/CCL2 and nitric oxide. Shock, 2013; 39:63–69.
32.
WoodS, JayaramanV, HuelsmannEJ, et al.: Pro-inflammatory chemokine CCL2 (MCP-1) promotes healing in diabetic wounds by restoring the macrophage response. PLoS One, 2014; 9:e91574.
33.
RussoRC, GarciaCC, TeixeiraMM, AmaralFA: The CXCL8/IL-8 chemokine family and its receptors in inflammatory diseases. Expert Rev Clin Immunol, 2014; 10:593–619.
34.
HaradaA, SekidoN, AkahoshiT, WadaT, MukaidaN, MatsushimaK: Essential involvement of interleukin-8 (IL-8) in acute inflammation. J Leukoc Biol, 1994; 56:559–564.
35.
NewtonK, DixitVM: Signaling in innate immunity and inflammation. Cold Spring Harb Perspect Biol, 2012; 4:pii: a006049.
36.
NatoliG, SaccaniS, BosisioD, MarazziI: Interactions of NF-kappaB with chromatin: The art of being at the right place at the right time. Nat Immunol, 2005; 6:439–445.
37.
ThompsonWL, Van EldikLJ: Inflammatory cytokines stimulate the chemokines CCL2/MCP-1 and CCL7/MCP-3 through NFkB and MAPK dependent pathways in rat astrocytes [corrected]. Brain Res, 2009; 1287:47–57.
38.
RovinBH, DickersonJA, TanLC, HebertCA: Activation of nuclear factor-kappa B correlates with MCP-1 expression by human mesangial cells. Kidney Int, 1995; 48:1263–1271.
39.
GoebelerM, GillitzerR, KilianK, et al.: Multiple signaling pathways regulate NF-kappaB-dependent transcription of the monocyte chemoattractant protein-1 gene in primary endothelial cells. Blood, 2001; 97:46–55.
40.
IslamMS, YoshidaH, MatsukiN, et al.: Antioxidant, free radical-scavenging, and NF-kappaB-inhibitory activities of phytosteryl ferulates: Structure-activity studies. J Pharmacol Sci, 2009; 111:328–337.
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