Herein, the probiotic potential of Blautia producta was investigated by whole-genome analysis. The genome assembly leads were successful at 100% completeness with 3.8% contamination. The leads comprised 120 contigs spanning 5,965,788 bp, with an average gene cluster of 45.91% and a high N50 value of 142,259 bp, indicating excellent assembly quality. A total of 215 subsystems related to metabolism, protein processing, and stress response were identified. Moreover, 4630 encodes nearly 64 functional proteins involved in transcription, carbohydrate transport, signal transduction, and defense. Kyoto Encyclopedia of Genes and Genomes and Clusters of Orthologous Group analyses also revealed pathways involved in nutrient transport, protein synthesis, and quorum sensing. The two unique gene clusters identified, the nonribosomal peptide synthetase and ranthipeptide clusters, are known for their antimicrobial and signaling activities. Furthermore, B. producta showed no resistance to 116 tested antimicrobial agents and had low (42.7%) human pathogenicity. In contrast to other probiotics, B. producta also has similar genomic and functional attributes, which support various research and therapeutic applications.
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