Magnetic Field and Ultrasound to Intensify the Production of Biomass,Polysaccharides and Enzymes by Lentinula edodes Cultivated in Submerged Fermentation

Abstract

The application of ultrasound and magnetic fields were tested as physical, clean and advanced technologies to obtain biomass, polysaccharides and enzymes from Lentinula edodes in submerged cultivation. The highest biomass concentration found was 13.65 ± 0.30 g L⁻¹, obtained by applying a magnetic field of 60 mT throughout the cultivation. In this same condition, the highest activities of endocellulase and exocellulase enzymes were to be 7.85 ≤ 0.01 U mL⁻¹ and 7.37 ≤ 0.01 U mL⁻¹, respectively. Using ultrasound for 10 min d⁻¹, the highest yield of polysaccharides (53.2% in weight) and the highest activity of laccase enzyme (2.08 ≤ 0.01 U mL⁻¹) were obtained. The application of physical field technologies demonstrates the enormous potential of Lentinula edodes in producing biomass, polysaccharides and enzymes as a biotechnologically cleaner alternative. In addition, these are green technologies that may be operated on an industrial scale to obtain these bioproducts.