Spirulina is an excellent source of proteins, vitamins, minerals, and essential fatty acids. Due to its nutritional composition, this microalga has been commercially produced and used in a variety of foods. Spirulina has also been studied in the production of fermented milk to enhance the viability of probiotics, but the use of Spirulina in freeze-dried yogurts has not been studied. This study seeks to evaluate the effects of Spirulina sp. LEB 18 in freeze-dried yogurts, with addition before fermentation and after freeze drying. The yogurts' chemical characteristics and lactic acid bacteria count remained stable after freeze drying. The fatty acids that presented higher concentration were palmitic, stearic, and oleic acid. Moreover, it is possible to affirm that the addition of Spirulina sp. LEB 18 provided significant antioxidant activity and increased protein concentration of freeze-dried yogurt. The freeze-dried yogurt with added Spirulina sp. LEB 18 presented an acceptability index of 82.4%, proving that it satisfies consumer needs and provides nutritional and health benefits.
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References
1.
MorellP, Piqueras-FiszmanB, HernandoI, FiszmanS. How is an ideal satiating yogurt described? A case study with added-protein yogurts. Food Res Int, 2015; 78:141–147.
2.
VenirE, Del TorreM, StecchiniML, et al.Preparation of freeze-dried yoghurt as a space food. J Food Eng, 2007; 80:402–407.
3.
YamaguchiSKF, KrebsCS, BertolliSL, CarvalhoLF. Liofilização de produtos lácteos: Uma revisão. Revista Espacios, 2017; 38:1–12.
4.
GahruieHH, EskandariMH, MesbahiG, HanifpourMA. Scientific and technical aspects of yogurt fortification: A review. Food Sci Human Wellness, 2015; 4(1):1–8.
5.
BeheshtipourH, MortazavianAM, HaratianP, DaraniKK. Effects of Chlorella vulgaris and Spirulina platensis addition on the viability of probiotic bacteria in yogurt and its biochemical properties. Eur J Food Res Technol, 2012; 235:719–728.
6.
BeheshtipourH, MortazavianAM, MohammadiR, et al.Supplementation of Spirulina platensis and Chlorella vulgaris algae into probiotic fermented milks. Compr Rev Food Sci F, 2013; 12:144–154.
7.
BarkallahM, DammakM, LouatiI, et al.Effect of Spirulina platensis fortification on physicochemical, textural, antioxidant and sensory properties of yogurt during fermentation and storage. LWT-Food Sci Technol, 2017; 84:323–330.
8.
MoraisMG, MirandaMZ, CostaJAV. Chocolate cookies enriched with Spirulina platensis: physical-chemical, sensory and digestibility characteristics. Alim Nutr Araraquara, 2006; 17:323–328.
9.
FradiqueM, BatistaAP, NunesMC, et al.Incorporation of Chlorella vulgaris and Spirulina maxima biomass in pasta products. Part 1: Preparation and evaluation. J Sci Food Agric, 2010; 90:1656–1664.
10.
SantosTD, FreitasBCB, MoreiraJB, et al.Development of powdered food with the addition of Spirulina for food supplementation of the elderly population. Innov Food Sci Emerg Technol, 2016; 37:216–220.
11.
CarvalhoLF, MoreiraJB, OliveiraMS, CostaJAV. Novel food supplements formulated with Spirulina to meet athletes' needs. Braz Arch Biol Technol, 2017; 60:e17160656.
12.
MoraisMG, RadmannEM, AndradeMR, et al. Pilot scale semicontinuous production of Spirulina biomass in southern Brazil, Aquaculture 2009;294:60–64.
13.
MadkourFF, KamilAE, NasrHS. Production and nutritive value of Spirulina platensis in reduced cost media, Egypt J Aquat Res 2012;38:51–57.
14.
FDA. Food and Agriculture Organization of the United Nations, Agency response letter. GRAS notice No. GRN 000127 6 October 2003, 2003.
15.
SoheiliM, Khosravi-DaraniK. The potential health benefits of algae and micro algae in medicine: A review on Spirulina platensis. Curr Nutr Food Sci, 2011; 7(4):279–285.
ÖzerBH, RobinsonRK. The behavior of starter cultures in concentrated yogurt (Labneh) produced by different techniques. LWT-Food Sci Technol, 1999; 32:391–395.
18.
SilvaN, Junqueira VCA and Silveira NFA. Manual de Métodos de Análise Microbiológica de Alimentos. São Paulo, SP: Editora Varela, São Paulo, São Paulo, 2010.
19.
AOAC. Official Methods of Analysis of the Association of Official Analytical Chenusts, ed. Maryland: Association of Official Analytical Chemists, 2000.
20.
CavinA, HostettmannK, DyatmykoW, PotteratO. Antioxidant and lipophilic constituents of Tinospora crispa. Planta med, 1998; 64:393–396.
21.
SaifyZS, AkhtarS, KhanKM, et al.A study on the acid composition of fisch liver oil from two marine Fish, Eusphyra blochii and Carcharhinus bleekeri. Turk J Chem, 2003; 27:251–258.
22.
StoneH, SidelJ. Sensory Evaluation Practices. San Diego, CA: Elsevier Academic Press, San Diego, California, 2004.
FernandesSS, Salas-MelladoMM. Addition of chia seed mucilage for reduction of fat content in bread and cakes. Food Chem, 2017; 227:237–244.
25.
FAO/WHO. Codex alimentarius: Codex standards for fermented milks. In: Milk and Milk Products, second ed. World Health Organization, Food and Agriculture Organization of the United Nations, Rome, 2011:6–16.
26.
GomesAMP, MalcataFX. Bifidobacterium spp. and Lactobacillus acidophillus: biological, biochemical, technological and therapeutical properties relevant for use as probiotics. Trends Food Sci Technol, 1999; 10:139–157.
27.
HongSH, MarshallRT. Natural exopolysaccharides enhance survival of lactic acid bacteria in frozen dairy desserts. J Dairy Sci, 2001; 84:1367–1374.
28.
MercenierA, PavanS, PotB. Probiotics as biotherapeutic agents: Present knowledge and future prospects. Curr. Pharm Des, 2003; 8:99–110.
29.
PerezKJ, GuarientiC, BertolinTE, et al.Viabilidade de bactérias láticas em iogurte adicionado de biomassa da microalga Spirulina platensis durante o armazenamento refrigerado. Alim Nutr Araraquara, 2007; 18:77–82.
30.
GyenisB, SzigetiJ, MolnarN, VargaL. 2005. Use of dried microalgal biomasses to stimulate acid production and growth of Lactobacillus plantarum and Enterococcus faecium in milk. Acta Agraria Kaposvariensis, 2005; 9(2):53–59.
31.
NiccolaiA, ShannonE, Abu-Ghannam, et al. Lactic acid fermentation of Arthrospira platensis (spirulina) biomass for probiotic-based products. J Appl Phycol, 2018; 1–7.
BeckerEW.Micro-algae as a source of protein. Biotechnol Adv, 2007; 25:207–210.
34.
AgustiniTW, Ma'rufF, Widayat, et al. Study on the effect of different concentration of Spirulina platensis paste added into dried noodle to its quality characteristics. IOP Conference Series: Earth and Environmental Science, 2017; 55(012068):1–9.
35.
CarvalhoLF, MoreiraJB, OliveiraMS, CostaJAV. Acceptance of novel foods for physical activity practitioners formulated with Spirulina. IOSR J Biotechnol Biochem, 2016; 2:7–11.
36.
MoreiraJB, TerraALM, CostaJAV, MoraisMG. Utilization of CO2 in semi-continuous cultivation of Spirulina sp. and Chlorella fusca and evaluation of biomass composition. Braz J Chem Eng, 2016; 33:691–698.
SinghP, SinghR, JhaA, et al.Optimization of a process for high fibre and high protein biscuit. J Food Sci Technol, 2015; 52(3):1394–1403.
39.
GoirisK, MuylaertK, FraeyeI, et al.Antioxidant potential of microalgae in relation to their phenolic and carotenoid content. J Appl Phycol, 2012; 24:1477–1486.
40.
IsmaielMM, El-AyoutyYM, Piercey-NormoreM.Role of pH on antioxidants production by Spirulina (Arthrospira) platensis. Brazil J Microbiol 2016:47(2):298–304.
41.
AnbarasanV, Kishor KumarV, Satheesh KumarP, VenkatachalamT. In vitro evaluation of antioxidant activity of blue green algae S. platensis. Int J Pharm Sci Res, 2011; 2(10):2616–2618.
42.
VonshakA.Spirulina platensis (Arthrospira):Physiology Cell-Biology and Biotechnology. Londres: Taylor & Francis, 1997.
43.
BhatVB, MadyasthaKM. C-phycocyanin: A potent peroxyl radical scavenger in vivo and in vitro. Biochem Biophys Res Commun, 2000; 275:20–25.
44.
BertolinTE, FariasD, GuarientiC, et al.Antioxidant effect of phycocyanin on oxidative stress induced with monosodium glutamate in rats. Braz Arch Biol Technol, 2011; 54:733–738.
MoraisMG, PratesDF, MoreiraJB, et al.Phycocyanin from microalgae: Properties, extraction and purification, with some recent applications. Ind Biotechnol, 2018; 14:30–37.
47.
CostaEL, GontijoJAR, NettoFM. Effect of heat and enzymatic treatment on the antihypertensive activity of whey protein hydrolysates. Int Dairy J, 2007; 17:632–640.
48.
HartmannR, MeiselH. Food-derived peptides with biological activity: From research to food applications. Curr Opin Biotechnol, 2007; 18:163–169.
49.
CostaJAV, RadmannEM, CerqueiraVS, et al.Perfil de ácidos graxos das microalgas Chlorella vulgaris e Chlorella minutissima cultivadas em diferentes condições. Alim Nutr Araraquara, 2006; 17:429–436.
50.
WillisWM, LenckiRE, MarangoniAG. Lipid modification strategies in the production of nutritionally functional fats and oils. Crit Rev Food Sci Nutr, 1998; 38:639–674.
51.
AgostiniC.Compliance of present recommendations of fatty acids in formulas for term infants with the actual human milk fatty acid composition in different populations, Acta Paediatr 2003;92:785–789.
52.
Gómez-CortésP, JuarézM, FuenteMA. Milk fatty acids and potential health benefits: An updated vision. Trends Food Sci Technol, 2018; 81:1–9.
53.
ThorningTK, BertramHC., BonjourJP, et al.Whole dairy matrix or single nutrients in assessment of health effects: Current evidence and knowledge gaps. Am J Clin Nutr, 2017; 105:1033–1045.
54.
Ruiz-NúñezB, Dijck-BrouwerDAJ, MuskietFAJ. The relation of saturated fatty acids with low-grade inflammation and cardiovascular disease. J Nutr Biochem, 2016; 36:1–20.
55.
SpeharCR, SantosRLB. Quinoa BRS Piabiru: alternative for diversification of cropping systems. Pesq Agropec Bras, 2002; 37:889–893.
56.
FigueiraFS, CrizelTM, SilvaCR, Salas-MelladoMM. Elaboration of gluten-free bread enriched with the microalgae Spirulina platensis. Braz J Food Technol, 2011; 14:308–316.
57.
LucasBF, MoraisMG, SantosTD, CostaJAV. Effect of Spirulina addition on the physicochemical and structural properties of extruded snacks. Food Sci Technol, 2017; 37:16–23.
