The global clean energy supply has drawn tremendous attention toward alternative resources for biofuel. Microalgae are proving a potential source due to their high oil content and rapid biomasss production, but it still challenging to find potential species and produce biomasss at low-cost. The present work describes the sucessful collection, isolation, and identification of Mexican native microalgae species from eutrophic water.Morphological studies were conducted by analyzing the size and shape of pyrenoids, cell walls, nuclei, and mucilages with the help of light microscopy, and molecular analysis was performed using the internal transcribed spacer (ITS, 18–28S rRNA) gene partial sequence followed by phylogenetic analysis. Three species isolated from eutrophic water (Chlorella vulgaris, Chlorella saccharophila and Scenedesmus dimorphus), two standard culture media (Bold's Basal Medium (BBM) and Murashige and Skoog), and partially treated wastewater (TEC) were used in this study to select native potential microalgae for low-cost biomass and lipid production. The biomasss and lipid accumulation was nearly similar between BBM and TEC. It was concluded that it is feasible to use wastewater (TEC) to cultivate these native algae to treat wastewater for removal of nutrients and produce lipid at a lower cost than standard medium. Furthermore, the native species can be used without seasonal acclimatization. It is necessary to conduct further experiments to optimize the culture condition and maximize biomasss production in order to scale the production to an industrial level.
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