In this study, solid-state fermentation was optimized for the production of chitinases by fungus Metarhizium anisopliae using sugarcane bagasse as substrate. A Central Composite Rotatable Design (CCRD) was used to determine the effect of temperature, moisture, and chitin mass in chitinase production. The highest value for the total chitinolytic activity was obtained at 33°C, chitin mass 0.75 g, and moisture content 60%.
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