This work aimed to optimize the process parameters for exo-polygalacturonase (exo-PG) recovery and production from Aspergillus niger ATCC 9642. Different types of solvents and extraction solutions (distilled water, NaCl, Tween 80, citrate buffer, and acetate buffer) have been evaluated for enzymes recovery using agitation (12 to 348 rpm), time (4.8 to 55.2 min), temperature (13.2 to 46.8°C), and extraction cycles (10 cycles). Our work's maximum exo-PG activity was 23 U/gwm(68 U/gdm), obtained after 192 h of bioproduction with 9 g orange peel, 4 g corn steep liquor, and 7 g wheat bran concentrations, at 30°C, and 5 × 106 spores/gwm. The maximum recovery was obtained using NaCl (0.1 mol/L) as solvent at a 5:1 (v:w) ratio, 30-55 min, 180 rpm, in one extraction cycle. Optimum pH and temperature for crude enzyme extract were 4.5 and 55°C, respectively.
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