DNA-Free CRISPR-Cas9 Gene Editing

Abstract

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Microinjection of zebrafish embryos using Dharmacon™ Edit-R™ Cas9 Nuclease mRNA and synthetic crRNA and tracrRNA for gene engineering. Blasky∗

A.J.

, Haas

, Schiel

J.A.

, Prekeris∗

, Kelley

M.L.

. ∗ University of Colorado, Denver, CO. Dharmacon, Lafayette, CO.