Persistence of Habitable,but Uninhabited,Aqueous Solutions and the Application to Extraterrestrial Environments

Abstract

In most environments on Earth, habitable environments contain life. Experiments were conducted to investigate the decoupling of the presence of habitable conditions and life. A set of microcosms habitable for known groups of organisms, but uninhabited (i.e., uninhabited habitats), was exposed to external environmental conditions to test the hypothesis that extreme habitable environments can remain uninhabited for sustained time periods. These microcosms were made of tubes containing liquid water and inorganic N, P, and S. Organics (used as electron donors and as a C source) were provided as L and D amino acids. One set of uninhabited habitats contained no additional salts, one set contained saturated NaCl, and one set contained saturated MgSO₄. A ddH₂O control and a complex medium for Halobacterium were used as controls. The presence of organisms was tested by enumeration of colonists and sequencing of extracted DNA. At each time point, inoculation into fresh medium was used to test for growth of organisms. After 1 week, the “no salt” and saturated MgSO₄ solutions were colonized. After 6 months, both the NaCl-saturated and Halobacterium solutions remained uninhabited, but all other samples were colonized. These experiments demonstrate that certain types of habitable liquid water environments exposed to microbial atmospheric inoculation, even on Earth, can remain devoid of reproducing life for many months. On other planetary bodies, such as Mars, these data imply the possibility of preserved transient water bodies that would record habitable conditions, but no evidence of life, even if life existed elsewhere on the planet.