Characterization of a Monoclonal Antibody Specific for a Novel Primate Cell Surface Marker with Distinct Biochemical Properties on Human Erythroleukemia and Myeloid Cell Lines

Abstract

A hybridoma, CSC-1, which secretes monoclonal antibody (MAb) specific for a cell surface molecule on African green monkey kidney cell line, BGMK, was isolated and characterized. The cell surface molecule recognized by CSC-1 is widely expressed on a variety of human cell lines. Among the hematopoietic cell lines examined, the CSC-1 marker seems to be preferentially expressed by lymphoid cell lines (e.g., Raji, CEM-SS, Jurkat, and MOLT-3). Although CSC-1 also recognizes some uncommitted myeloid and erythroleukemia cell lines (e.g., U-937 and K562), the CSC-1 marker expression on these cell lines is subjected to PMA-induced down-regulation. In addition, the PMA-induced cell surface down-regulation of the CSC-1 marker required more than 24 h. Proteolytic analysis shows that the CSC-1 marker can be categorized into proteinase K-resistant and-sensitive phenotypes. Furthermore, the CSC-1 marker exhibits a slow cell surface turnover rate after proteinase K treatment. Our data suggest that the CSC-1 cell surface molecule might be useful as a megakaryocytic or monocytic differentiation marker.