Potentiation of Antimetastatic Activity of Pentoxifylline in B16F10 and B16F1 Melanoma Cells Through Inhibition of Glutathione Content

Abstract

Purpose: The aim of this work is to evaluate the antioxidative activity of antimetastatic agent pentoxifylline (PTX) in B16F10 and B16F1 melanoma cells. Materials and methods: B16F10 and B16F1 cell lines were treated with PTX with different doses at different time intervals. Reduced glutathione, glutathione-S-transferase, and lipid peroxides were estimated to evaluate the action of PTX. Results: The activity of pentoxifylline treatment showed glutathione depletion, increased glutathione-S-transferase activity with upregulation of lipid peroxidation in B16F10 and B16F1 melanoma cells. However, the effect on GSH, GST, and LPOs was dependent on dose concentrations and time intervals. Conclusions: Pentoxifylline, an effective membrane modifier, showed significant depletion in glutathione level in conjunction with increased lipid peroxidation. The results suggested an antimetastatic property by PTX at a nontoxic dose in B16F10 and B16F1 melanoma cells.