Time-Dependent Changes in Rat Brain Cholinergic Receptor Expression after Experimental Brain Injury

Abstract

Alterations in neurotransmitter receptor expression in the central nervous system may contribute to physiological and behavioral deficits that follow traumatic brain injury (TBI). Previous studies from our laboratory have demonstrated significant and widespread deficits in α7* nicotinic cholinergic receptor (α7* nAChr) expression 2 days following cortical contusion brain injury. The purpose of this study was to evaluate changes in α7* nAChr expression over a wider range of post-TBI recovery intervals. Animals were anesthetized and subjected to a moderate cortical contusion brain injury(2 mm cortical compression). Animals were euthanatized at various post-TBI time intervals, ranging from 1 h to 21 days, and quantitative autoradiography was used to evaluate cholinergic receptor subtype expression in the cerebral cortex and hippocampus. As previously reported, the α7* nAChr was the most sensitive target of TBI-induced plasticity. Significant decreases in α-[¹²⁵I]-bungarotoxin(BTX) binding occurred as early as 1 h post-TBI, and persisted in some brain regions for up to 21 days. A kinetic analysis of changes in BTX binding, performed 2 days following brain injury, indicated that the binding deficits are not due to significant changes in receptor affinity. TBI-induced changes in α3*/α4* nACh receptors, muscarinic cholinergic receptors, and NMDA-type glutamate receptor expression were lower in magnitude, restricted to fewer brain regions and more transient in nature. Persistent deficits in α7* nAChr expression following TBI may contribute to impaired functional outcome following brain injury.