Human hepatic carbohydrate metabolism

Abstract

Purpose: Dynamic natural-abundance ¹³C MR spectroscopy (MRS) studies without proton decoupling were performed in the human liver using commercial 1.5 T MR equipment

Material and Methods: A single tuned custom-made circular surface coil with an OD of 20 cm operating at 16.04 MHz was used for the ¹³C study. Seventy-five grams of glucose dissolved in water was administered for the natural-abundance ¹³C-MRS dynamic study which lasted for approximately 40 to 60 min. Data acquisition was broken into 20-min and 1.7-min blocks. Localized proton shimming with a whole-body coil was performed with sufficient volume to include the observing area of the surface coil; the line width of the water signal was less than 20 Hz

Results and Conclusion: the glucose and glycogen spectra were clearly visible at 80 to 120 ppm after oral administration of the glucose solution. These data demonstrate that dynamic hepatic carbohydrate metabolism can be observed with commercially available MR equipment. Given that the human hepatic glycogen pool reaches maximum level within less than 10 min, this technique should provide a direct diagnosis of hepatic carbohydrate metabolic disorders