This experiment evaluated effects following chronic toxicity with 20 ppm fenvalerate (synthetic pyrethroid), 2 ppm monocrotophos (organophosphate) and 2 ppm endosulfan (chlorinated hydrocarbon) on bone health of broiler chicks. A total of 120 chicks were divided equally into 4 groups and were fed poultry mash without (control) or mixed with different pesticides for 8 weeks. Body mass, serum calcium and phosphorus levels were unaffected due to pesticides treatment. However, an increase (p < 0.01) in serum alkaline phosphatase activity was noted and serum total protein decreased (p < 0.01) in all treated groups. Roentogenography revealed destructive changes in the upper part of the femur in the monocrotophos group. Endosulfan intoxicated chicks had increased numbers of trabeculae in the medullary cavity. Microscopic alterations of the costochondral junction in intoxicated chicks were similar. The zones of proliferating, maturing and degenerating, and calcifying cartilage cells were reduced in width and the metaphysis in treated birds showed a reduced number of cartilage cells and thinner trabeculae. Due to toxicity, the capillary scaffolding of the degenerating cartilage cells was reduced and a larger number of transverse trabeculae could be seen in the metaphysis. Appositional bone growth studied by the tetracyclicline labeling technique indicated decreased active osteons.
Asling, C.W., Tse, F., and Rosenberg, L.L. (1968). Effect of growth hormone and thyroxine on sequence of chondrogenesis in the epiphyseal cartilage plate. In Growth Hormones (A. Pecile and E. E. Muller, eds.). Proceedings of the First International Symposium. Int congress series No 158. Excerpta Medica Foundation, Amsterdam/ New York, pp. 319—31.
2.
Beard, J., Marshall, S., Jong, K., Newton, R., Triplett-McBride, T., Humphries, B., and Bronks, R. ( 2000). 1,1,1-trichloro-2,2-bis (p-chlorophenyl)-ethane (DDT) and reduced bone mineral density. Arch Environ Health55,177—80.
3.
Biogin, E., Shimuhony, A., Avidar, Y., and Iseraeli, B. (1981). Enzymes metabolites and electrolytes in blood of Israeli goats. Zentrab Vet Med A28, 135—40.
4.
Compston, J.E., Vedi, S., Stephen, A.B., Bord, S., Lyons, A.R., Hodges, S.J., and Scammell, B.E. (1999). Reduced bone formation after exposure to organophosphates . Lancet354, 1791—2.
5.
Cooper, M.D., Peterson, R.D.A., South, M.A., and Good, R.A.( 1966). The functions of the thymus system and the bursa system in the chickens. J Exp Med123, 75—102.
6.
Falconer, W.R., and King, J.W. (1970). Manual of Clinical Laboratory Procedures. 2nd ed. The Chemical Rubber Company, Cleveland, OH.
7.
Frost, H.M. (1958). Preparation of thin uncalcified bone section by rapid manual method. Stain Technol33, 273—7.
8.
Frost, H.M. (1964). Dynamics of bone remodeling In Bone Biodynamics (J. A. Frost, ed.), pp. 315—33, Churchill Ltd., London.
9.
Gilman, A.G., Goodman, L.S., and Gilman, A. (1980). The Pharmacological Basis of Therapeutics. 6th ed. Macmillan, New York.
10.
Gomori, G.A. (1942). Modification of the colorimetric phosphorus determination for the use with the photoelectric colorimeter. J Lab Clin Med27, 955—60.
11.
Hall, T.J., Nyugen, H., Shaueblin, M., and Fournier, B. (1995). The bonespecific estrogen centchroman inhibits osteoclastic bone resorption in vitro. Biochem Biophys Res Comm216, 662—8.
12.
Hoffman, D.J., Sileo, L., and Murray, H.C. (1984). Sub-chronic organophosphorus ester-induced delayed neutrotoxicity in mallards. Toxicol Appl Pharmacol75, 128—36.
13.
Hudson, H.A., Britton, W.M., Rowland, G.N., and Buhr, R.J. (1993). Histomorphometric bone properties of sexually immature and mature White Leghorn hens with evaluation of fluorochrome injection on egg production traits. Poult Sci72, 37—47.
14.
Jefferies, D.J., Franch, M.C., and Osborne, B.S. (1971). The effect of P'P'-DDT on the rate amplitude and weight of the heart of pigeon and Bengalese finch. Br Poult Sci12, 387—99.
15.
Kaneko, J.J. (1989). Clinical Biochemistry of Domestic Animals. 4th ed., p. 886. Academic Press, New York.
16.
Kind, P.R.M., and King, E.J. (1954). Serum acid and alkaline phosphate activity. J Clin Path7, 322.
17.
Kolaja, G.J., and Hinton, D.E. (1978). Ultrastructural alteration in the egg shell gland epithelium of the mallard duck after chronic exposure to DDT. Environ Pollut7, 237—44.
18.
Ludle, J.L., Mehrle, M.P., Foster, L.M., and Earlkaiser, T. (1979). Bone development in black ducks as affected by dietary toxophene. Pestic Biochem Physiol10, 168—73.
19.
Majumdar, S., Chakraborty, A.K., Mandal, K., Bhattacharya, A., and Basak, D.K. (1994). Subacute toxicity in broiler chick: Concentration cytotoxicity and biochemical profile. Indian J Expt Bio32, 752—6.
20.
Manciuela, S., and Giurgea, R. (1975). Effect of small doses of insecticides on the liver of chicks. Zentrlbl Veterinaer Med A22, 671—7.
21.
Matos, E., Loria, D., Daedo, A., and Lusting, E.S. (1985). Effects of pesticides and 12-0-tetra dicanoylphorbol-13-acetate on chondrogenesis. In vitro Biol4, 175—84.
22.
Meneely, G.A., and Wyttenbach, C.R. (1989). Effects of organophosphate insecticide diazinon and parathion on bobwhite quail embryos: skeletal defects and acetylcholinesterase activity. J Expt Zool252, 60— 70.
23.
Misawa, M., Doull, J., and Uyeki, E.M. (1982). Teratogenic effects of cholinergic insecticides in chick embryos. III Development of cartilege and bone. J Toxicol Environ Health10, 551—63.
24.
Muller, H.D., and Lockman, D.C. (1973). The influence of dieldrin on tibial bone calcium deposition in domestic chicks. Bull Environ Contam Toxicol9, 351—5.
25.
Pal, A.K., and Kushwah, H.S. (1990). In vitro action of malathion of certain metabolic enzymes. J Vet Physiol AlliedSci9, 1—8.
26.
Pal, A.K., Kushwah, H.S., and Kushwah, A. (1990). Impact of Malathion dipping one some carbohydrate constituents in poultry birds. Indian Vet Med J15, 104—7.
27.
Pal, A.K., and Kushwah, H.S. (1998). An assay of brain acetylecholinesterase activity vis-à-vis malathion toxicity in chicken. Indian Vet Med J22, 233.
28.
Pal, A.K., and Kushwah, H.S. (2000). Quantative biological lesions of malathion dipping in domestic fowl Gallus domesticus. Asian-AustraJ Anim Sci13(Suppl), 285—90.
29.
Pushpanjali, Pal , A.K., and Prasad, R.L. (1999). An avian model for the study of in vivo embryotoxicity of endosulfan: determination of LD50 and influence on ponderal changes Indian VetMed J23, 321—2.
30.
Rahman, M.F., Siddiqui, M.K., and Jamil, K. (2000). Acid and alkaline phosphatase activities in a novel phosphorothionate (RPR-11) treated male and female rats: evidence of dose and time-dependent response. Drug Chem Toxicol23, 497—509.
31.
Reyes, M.D., and Moore, E.C. (1979). Tissue residues and ultrastructural changes induced by DDT in chickens. Poult Sci58, 1183—91.
32.
Sanhotra, M.K., Kushwah, H.S., Pal, A.K., and Datta, I.C. (1996). Effect of endosulfan upon phosphomonoesterase and transaminase activity in rat erythrocytes. Indian Vet J73, 833—5.
33.
Singh, B.P., Singhal, L.K., and Chauhan, R.S. (2001). Down regulation of macrophage functions in chickens due to fenvalerate contaminated feed. The 10th International Conference of the Association of Institutions for Tropical Veterinary Medicine “Livestock Community and Environment” 20—23 August 2001Copenhagen Denmark 〈http://www.aitvmkv1dk/Abstracts/poster_session_jhtm〉 accessed on date 05/07/02).
34.
Singh, K.K., and Jha, G.J. (1982). Bone growth during chronic aldrin intoxication in goats. Res Vet Sci32, 282—8.
35.
Smith, G.J. (1993). Toxicology and pesticide use in relation to wildlife: Organophosphates and Carbamate compounds. C. K. Smoley , Boca Raton, FL.
36.
Snedecor, G.W., and Cochran, W.C. (1968). Statistical Method. 7th ed. Oxford and IPH Publishing Co., New Delhi.
37.
Soto, A.M., Chung, A.L., and Sonnenschein, C. (1994). The pesticides endosulfan toxaphene and dieldrin have estrogenic effects on human estrogen-sensitive cells. Environ Health Perspect102, 380—3.
38.
Stuart, A.J., and Smith, D.A. (1992). Use of fluorochromes xylenol orange calcein green and tetracycline to document bone deposition and remodelling in healing fractures in chickens. Avian Dis36, 447—9.
39.
Svendsen, O. (1973). Ultrastructure of livers from chickens embryogenically injected with DDT. Acta Pharmacol Toxicol (Copnh)33, 113—22.
40.
Tamang, R.K., Jha, G.J., Gupta, M.K., Chauhan, H.V., and Tiwary, B.K. (1988). In vivo immunosuppression by synthetic pyrethroid (cypermethrin). pesticide in mice and goatsVet Immunol Immunopathol19, 299—305.
41.
Tamura, H., Yoshikawa, H., Gaido, K.W., Ross, S.M., DeLisle, R.K., Welsh, W.J., and Richard, A.M. (2003). Interaction of organophosphate pesticides and related Compounds with the androgen receptor. Environ Health Perspect111, 545—52.
42.
Tayyaba, K., Hasan, M., Islam, F., and Khan, N.H. (1981).Organophosphate Pesticide metasystox induced regional alternation in brain nucleic acid metabolism. Indian J Exp Biol19, 688—90.
43.
Trinder, P. (1960). Determination of serum calcium. Analyst85, 889.
44.
Varshneya, C., Bahga, H.S., and Sharma, L.D. (1988). Toxicological effects of dietary endosulfan in cockerels. Indian J Poult Sci23, 4—8.
45.
Weischelbaum, T.E. (1946). Determination of protein. Am J Clin Path16, 40.
