We described previous that the adrenal medulla recovers rapidly from the injuries due to salinomycin. In the present study we found the recovery to be apparently due to differentiation of pre-existing, primitive undifferentiated, chromaffi n cells, rather than mitosis, as evidenced by lack of incorporation of 5-bromo-2-deoxyuridin e in the differentiating and/or differentiated chromaffi n cells. Electron-dense, striated fi brils observed within the necrotic cells and in the extracellular space were presumed to be intermediate degradation products. Schwann cells actively functioned as phagocytes in this study, and seemed to indirectly stimulate the differentiation of undifferentiated chromaffi n cells.
Chen-Pan C., Pan IJ, Yamamoto Y., Sakogawa T., Yamada J., Hayashi Y. (1999). Prompt recovery of damaged adrenal medullae induced by salinomycin. Toxicol Pathol27: 563—572.
2.
Coupland RE, Kobayashi S., Serizawa Y., Fujita T. (1979). SGC cell: The third type of adrenal chromaffi ncell. In: Catecholamines: Basic and Clinical Frontiers. Usdin E, Kopin U , Barchas J (eds). Pergamon Press, New York, pp 313—315.
3.
Ewing SE, Weber RJ, Zauner A., Plunkett RJ (1992). Recovery in hemiparkinsonian rats following intrastriatal implantation of activated leukocytes. Brain Res576: 42—48.
4.
Fiandaca MS, Kordower JH, Hansen JT, Jiao S., Gash DM ( 1988). Adrenal medullary autografts into thebasal gangli a of cebus monkeys injury-induced regeneration. Exp Neurol102: 76—91.
5.
Goldfi en A. (1991). Adrenal medulla. In: Basic and Clinical Endocrinology, Greenspan FS (ed). Appleton and Lange, East Norwalk, Connecticut, pp 380—399.
6.
Grothe C., Hofmann HS, Verhofstad A., Unsicker K. (1985). Nerve growth factor and dexamethason e specify the catecholaminergi c phenotype of cultured rat chromaffi n cells: Dependence on developmental stage. Devl Brain Res21: 125—132.
7.
Heumann R., Lindholm D., Brandtlow C., Meyer M., Radeke MJ, Misko TP, Shooter EM, Thoenen H. (1987). Differential regulation of mRNA encoding nerve growth factor and its receptor in rat sciatic nerve during development degeneration and regeneration, role of macrophages. Proc Natl Acad Sci USA84: 8735—8739.
8.
Kikuta A., Ohtani O., Murakami T. (1991). Three-dimensional organization of the collagen fi brillar framework in the rat adrenal gland. Arch Histol Cytol54: 133—144.
9.
Mann HR, Whitney DR (1947). On a test of whether one of two random variables is stochastically larger than the other. Ann Math Stat18: 50—60.
10.
Mü ller TH, Unsicker K. (1986). Nerve growth factor and dexamethasone modulate synthesis and storage of catecholamines in cultured rat adrenal medullary cells: Dependence on postnatal age. J Neurochem46: 516—524.
11.
Schutte B.Reynders MMJ, Bosman FT, and Blijham GH (1987). Effect of tissue fi xation on anti-bromodeoxyuridin e immunohistochemistr y. J Histochem Cytochem35: 1342—1345.
12.
Unsicker K., Seidl K., Hofmann HD (1989). The neuro-endocrine ambiguity of sympathoadrenal cells. Int J Devl Neurosc7: 413—417.
13.
van der Rest M., Garrone R. (1991). Collagen family of protein . FASEB J5: 2814—2823.
14.
van Rooijen N., Kors N., Van Nieuwmegen R. (1984). Double immunocytochemical staining in the study of antibody-producin g cells in vivo. Detection of specifi c antibody-producin g cell in the spleen and simultaneous detection whether or not they produce immunoglobuli n G antibodies . JHistochem Cytochem32: 677—680.
15.
Unsicker K. (1976). Chromaffin, small granule-containing and ganglion cells in the adrenal gland of reptiles. A comparative ultrastructural study. Cell Tissue Res165: 477—508.
16.
Willems Iemg , Arends JW, Daemen Mjap (1996). Tenasin and fi bronectin expression in healing human myocardial scars. J Pathol179: 321—325.
