To characterize genetic alterations occurring in renal tumorigenesis, EHEN-induced renal cell tumors were examined using restriction landmark genomic scanning (RLGS) analysis, an electrophoretic separation technique that detects gene amplifi cations and deletions. Comparison of DNAs from tumor against those from corresponding nontumorous kidney and /or EHEN-treated kidney without development of renal tumors yielded specific alterations in terms of both amplifi ed and reduced DNA spots. Two amplifi ed spots were detected only in renal cell tumors and an additional four spots were frequent in EHEN-treated kidneys. One reduced spot was common to all tumor samples, and another was frequently detected in the tumors analyzed but not in EHEN-treated kidneys. A subset of the altered spots thus appeared to be specific for EHEN-induced renal tumorigenesis.
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