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Abstract

OBJECTIVE: This study determines the role of changes in numerical densities of myonuclei and satellite cells in age-related remodeling of the thyroarytenoid muscle (TA).

DESIGN: Changes in numerical densities (N_V) and ratios (N_N) of myonuclei and satellite cells were estimated for the entire TA by use of stereological techniques.

RESULTS: There was no age-related change or difference between fiber types for N_{V myonucleus, fiber'} but N_{v myonucleus, fiber} increased with decreasing fiber diameter. There was a trend toward a decrease in N_{v satellite cell, fiber} and a decrease in N_{N satellite cell, myonucleus.} N_{v satellite cell, fiber} was higher for type 1 than for type 2 fibers, and type 1 satellite cells increased disproportionately with increasing total satellite cell numerical density.

CONCLUSION: Decreased satellite cell proliferation may contribute to age-related fiber loss and atrophy in the TA.

SIGNIFICANCE: Therapeutic techniques based on activation of satellite cells may block age-related fiber loss and atrophy in the TA. (Otolaryngol Head Neck Surg 2000;123:377-84.)

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Numerical Densities of Myonuclei and Satellite Cells in Muscle Fiber Types in the Aging Human Thyroarytenoid Muscle: An Immunohistochemical and Stereological Study Using Confocal Laser Scanning Microscopy

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