The aim of this study was to determine the contribution of the human major histocompatibility complex (HLA)-DQA1, -DQB1, and TNFΑ genes with simple nasal polyposis.
Study Design and Setting
A comparative case-control study with 31 patients and 151 controls was performed. HLADQA1, -DQB1, and TNFΑ −238 promoter position loci were typed by polymerase chain reaction (PCR) and sequence specific oligonucleotide probes (SSOPs). TNFΑ ℒ308 promoter position was determined by PCR and digestion with NcoI restriction enzyme.
Results
The allele HLA-DQA1*0201 (Pc = 0.019) had an etiologic fraction (EF) of 17%, whereas 13% EF was found for the haplotype HLA-DQA1*0201-DQB1*0201 (P = 0.016). Analysis of -DQB1 and TNFΑ promoter did not show significant differences between cases and controls.
Conclusions
HLA-DQA1*0201-DQB1*0201 haplotype is involved in susceptibility, conferring 5.53 times more risk of developing this disease.
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