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Abstract

OBJECTIVE: Determine the sequence of gross and histopathologic change to the normal middle ear (ME), tympanic membrane (TM), and external auditory canal (EAC) during spontaneous gerbilline cholesteatoma development.

STUDY DESIGN AND SETTING: Sixty-six gerbils were examined weekly and periodically sacrificed for analysis.

RESULTS: Cholesteatoma development followed this sequence: 1) slightly thickened pars flaccida (PF) without ME effusion, 2) thickened PF with ME effusion, 3) continuous buildup of EAC debris, and 4) complete occlusion of the lateral EAC. In the cholesteatoma group, keratinizing epithelium (KE) thickness was increased in all regions over normal controls and regional gradations in the TM remained the same.

CONCLUSION: Spontaneous cholesteatoma formation was significantly associated with middle ear effusion, a failure to clear canal debris with resultant retrograde thickening of the EAC, PF, and then pars tensa.

SIGNIFICANCE: Inflammatory insult and alteration of the epithelial clearance mechanism appears to result in spontaneous cholesteatoma formation in gerbils as hypothesized for humans.

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References

Greenberg

Manolidis

High incidence of complications encountered in chronic otitis media surgery in a U.S. metropolitan public hospital. Otolaryngol Head Neck Surg 2001; 123:623–7.

Chole

Tinling

Inflammatory disease of the middle ear and their sequelae. In: Friedmann

Arnold

, eds. Pathology of the ear. London: Churchill Livingstone; 1993. p. 752.

Chole

Gerbil model of aural cholesteatoma. In: Cholesteatoma and Mastoid Surgery: Proceedings of the Fourth International Conference, Niigata, Japan. Kugler and Ghedini Publications, Amsterdam, Berkeley, Milano. 1992. 367–9.

Henry

Chole

McGinn

Age-related increase of spontaneous aural cholesteatoma in the Mongolian gerbil. Arch Otolaryngol 1983;109:19–21.

McGinn

Henry

Chole

Cholesteatoma: Experimental induction in the mongolian gerbil. Acta Otolaryngol 1982;93:61–7.

McGinn

Chole

Henry

Cholesteatoma induction: Consequences of external auditory canal ligation in gerbils, cats, hamsters, guinea pigs, mice and rats. Acta Otolaryngol 1984;97:297–304.

Chole

Tinling

Basal lamina breaks in the histogenesis of cholesteatoma. Laryngoscope 1985;95:270–5.

Wolfman

Chole

Experimental retraction pocket cholesteatomas. Ann Otol Rhinol Laryngol 1986;95:639–44.

Aminpour

Tinling

Brodie

Role of tumor necrosis factor-α sensorineural hearing loss after bacterial meningitis. Otol Neurotol 2005;26:602–9.

10.

Ratnesar

Chronic ear disease along the coasts of Labrador and Northern Newfoundland. J Otolaryngol 1976;5:122–30.

11.

Tos

Incidence, etiology and pathogenesis of cholesteatoma in children. Adv Otorhinolaryngol 1988;40:110–7.

12.

Fulghum

Chole

Bacterial flora in spontaneously occurring aural cholesteatomas in Mongolian gerbils. Infect Immun 1985;50:678–81.

13.

Fulghum

Chole

Brinn

. Mongolian gerbil tympanic membrane: Normal and with induced otitis media. Arch Otolaryngol 1987;113:521–5.

14.

Fortuny

Palomar

Nogues

Bacterial flora in the external ear canal and in induced cholesteatoma in the mongolian gerbil. Ann Otol Rhinol Laryngol 1993;102:559–61.

15.

Piepergerdes

Kramer

Behnke

Keratosis obturans and external auditory canal cholesteatoma. Laryngoscope 1980;90:383–91.

16.

Chole

McGinn

Tinling

Pressure induced bone resorption in the middle ear. Ann Otol Rhinol Laryngol 1985;94:165–70.

17.

Orisek

Chole

Pressures exerted by experimental cholesteatomas. Arch Otolaryngol 1987;113:386–91.

18.

Chole

Chan

Rapid induction of localized bone resorption in the auditory bulla of the Mongolian gerbil, Meriones unguiculatus, by increased air pressure. Calcif Tissue Int 1989;45:318–23.

Gerbilline Cholesteatoma Development. Part II: Temporal Histopathologic Changes in the Tympanic Membrane and Middle Ear

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