Objective

The objective of the study was to evaluate the antibacterial and antibiofilm efficacy of three essential oils (EOs), two derived from Tetraclinis articulata and one from Cedrus atlantica, against selected endodontic pathogens including Enterococcus faecalis, Streptococcus mitis, Streptococcus sanguinis, and Streptococcus gordonii.

Methods

The three EOs were extracted by means of hydrodistillation. The antibacterial activities expressed through Minimum Inhibitory Concentrations (MICs) and Minimum Bactericidal Concentration (MBCs), were evaluated in the microdilution assay. The biofilm inhibition and the biofilm disruption were tested for T. articulata₂ EO in crystal violet assay.

Results

The total yield of C. atlantica, T. articulata₁ and T. articulata₂ obtained was 0.1%, 0.2% and 0.45%, respectively. The MIC values of C. atlantica and T. articulata₁ EOs were above 20 mg/mL, while the MIC values of T. articulata₂ ranged from 4.78 ± 1.48 to 19.43 ± 5.48 mg/mL. Among the isolates, S. gordonii exhibited the highest sensitivity, while E. faecalis was the least sensitive. All tested concentrations (MIC/8–MIC) of T. articulata₂ EO successfully inhibited biofilm formation of the endodontopathogenic biofilms, with inhibition ranging from 34.91% to 78.90%. T. articulata₂ EO also proved disruptive potential on pre-formed biofilm of S. sanguinis and S. mitis at all tested concentrations.

Conclusion

T. articulata₂ proved promising antibacterial and antibiofilm potential which may be considered in dentistry.

Search results

Antibacterial and Antibiofilm Potential of Cedrus atlantica and Tetraclinis articulata Essential Oils Against Enterococcus faecalis and Three Streptococcus sp . Isolates for Endodontic Use: An in Vitro Study

Search results

Antibacterial and Antibiofilm Potential of Cedrus atlantica and Tetraclinis articulata Essential Oils Against Enterococcus faecalis and Three Streptococcus sp . Isolates for Endodontic Use: An in Vitro Study