The aim of this study was to screen specific adherent matrix for endothelial
progenitor cells (EPCs), which can be used for antibody capturing
stents.
Methods
In this study, the adhesion of EPCs on different matrices containing three
different antibodies, VEGFR-2, CD34, CD133, was observed under shear stress
in a flow chamber. Nitric oxide (NO) release, cell proliferation and the
retention rate of EPCs, were measured separately.
Results
The results demonstrated that shear stress within a certain range can promote
proliferation and NO secretion of EPCs. Under the same shear stress, the
EPCs showed stronger adhesion on matrix-containing CD133 antibody than on
the other matrices.
Conclusions
CD133 antibody has the potential application for EPCs capture.
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