Electro-Acupuncture (EA) Induced Attenuation of Immunosuppression Appearing after Epidural or Intrathecal Injection of Morphine in Patients and Rats

Abstract

In order to investigate the effect of epidural morphine and electroacupuncture (EA) on immune function of postoperative patients, peripheral blood for NK (natural killer) cell activity assay were obtained from patients before and on the 1st, 3rd, 7th day after cholecystectomy, lmg morphine injected into epidural space after surgery significantly suppressed NK cell activities via prolonged inhibition and increased the inhibitory intensity of it. EA of“ Zusanli (St.36), Sanyinjiao(Sp.6), Hegu(L.1.4) and Neiguan(P.6) points enhanced the NK cell activities after epidural injection of morphine. It was further observed that microinjection of 1 μ g/1 μ I naloxone into periaqueductal gray (PAG) patially antagonized the suppression of NK cell activity induced by intrathecal (ith) injection of 40 μ g/50 μ I morphine, and so does EA of “Zusanli(St.36)”. When EA applied after naloxone microinjectcd into PAG, the NK cell activity was further augmented as compared to that using EA or PAG microinjection of naloxone alone. The above results suggested epidural morphine combined with EA is a better method to relieve postoperative pain, and opiate receptor in PAG might partially mediate the suppression of NK cell activity induced by intrathecal injection of morphine. Yet, the mechanisms of the attenuation of EA on morphine induced immunosuppression needs further study.

Keywords

Epidural morphine. Intrathecal morphine Natural killer (NK) cell activity Cholecystectomy Electroacupuncture Naloxone Periaqueductal gray (PAG)

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