Curcumin supplementation and motor-cognitive function in healthy middle-aged and older adults

Abstract

BACKGROUND:

Recent studies suggest curcumin is a promising nutraceutical for improving important clinical and physiological markers of healthy aging, including motor and cognitive function.

OBJECTIVE:

To determine if curcumin supplementation improves motor and cognitive function in healthy middle-aged and older adults.

METHODS:

39 healthy men and postmenopausal women (45–74 yrs) were randomized to 12 weeks of placebo (n = 19) or curcumin supplementation (2000 mg/day Longvida^®; n = 20) with motor and cognitive function assessed at week 0 and 12.

RESULTS:

Using measures of the NIH Toolbox and other standardized tests, there were no changes in muscle strength and rate of torque development, dexterity, fatigability, mobility, endurance, and balance between the placebo and curcumin groups after 12 weeks (all P > 0.05). Additionally, there were no changes after 12 weeks of placebo and curcumin supplementation in measures of fluid cognitive ability, a cognitive domain that declines with age, including processing speed, executive function, working memory, and episodic memory (all P > 0.3). There were marginal changes in language, a measure of crystallized cognitive ability that is stable with age, following the intervention, wherein reading decoding increased 3% in the curcumin group (post: 2428±35 vs. pre: 2357±34, P = 0.003), but was unchanged in the placebo group (post: 2334±39 vs. pre: 2364±40, P = 0.07).

CONCLUSIONS:

Overall, 12 weeks of curcumin supplementation does not improve motor and cognitive functions in healthy middle-aged and older adults. It is possible that curcumin may enhance these functions in groups with greater baseline impairments than those studied here, including adults greater than 75 years of age and/or patients with clinical disorders.

Keywords

Aging curcumin motor cognitive NIH toolbox

1 Introduction

Advancing age is the primary risk factor for chronic degenerative diseases, disability, and mortality [1 –3]. Antecedent and contributing to this is a decline in physiological function, including motor and cognitive performance, which is not only an index of quality of life but predictive of future risk of morbidity and mortality [4 –8]. With the number of older adults over the age of 65 in the U.S. expected to double by the year 2050 to 25% of the population [9], strategies to extend healthspan - the number of years of independent and healthy living - are critically needed. Interventions, including nutraceuticals, represent possible strategies to enhance physiological function, including motor and cognitive performance, in middle-aged and older adults.

Curcumin, the active ingredient in the Indian spice turmeric, is a promising nutraceutical for improving important clinical and physiological markers of healthy aging. Recently, our laboratory showed that 12 weeks of curcumin supplementation improves vascular endothelial function in healthy middle-aged and older adults by reducing oxidative stress and increasing vascular nitric oxide bioavailability [10]. Oxidative stress, reduced nitric oxide bioavailability, and vascular endothelial dysfunction occur systemically and contribute to multiple physiological impairments and chronic diseases with aging [11 –14]. Accumulating evidence suggests that these processes may be determinants of age-related motor and cognitive dysfunction [13 , 15–17]. Consistent with this concept, work from our laboratory has shown that nutraceutical interventions that reduce oxidative stress, increase nitric oxide bioavailability, and improve vascular endothelial function also have potential to improve motor and cognitive function [18, 19].

Accordingly, the purpose of this study was to determine the efficacy of curcumin supplementation to improve motor and cognitive function in healthy middle-aged and older adults. To test this hypothesis, we performed a double-blind, parallel design, randomized study in which participants with baseline vascular endothelial dysfunction but free of physical or cognitive impairment received placebo or curcumin (2000 mg/day, Longvida^®) supplementation for 12 weeks, as an ancillary to vascular function outcomes. We utilized the National Institutes of Health (NIH) Toolbox motor and cognitive batteries, as part of the NIH Toolbox for the Assessment of Neurological and Behavioral Function [20], in combination with other standardized motor and cognitive measures to comprehensively assess multiple subdomains of function in a time-efficient manner. Several subdomains of motor function that decline with advancing age were assessed, including muscle strength and rate of torque development [21], dexterity [22], fatigability [23], mobility [24], endurance [25], and balance [26]. Fluid cognitive ability, a domain that comprises problem solving and the ability to encode episodic memories (processing speed, executive function, working memory, and long-term memory), and which declines with aging [27, 28], also was assessed. Finally, crystalized cognitive ability, a domain that measures declarative knowledge such as vocabulary and is relatively stable with age [27, 28], also was evaluated.

2 Materials and Methods

2.1 Participants

Thirty-nine healthy men and postmenopausal women aged 45 to 74 years from Boulder County, Colorado and the surrounding area were studied. All participants were non-smokers, free of cognitive impairment as assessed by the Mini Mental Status Exam (MMSE > 23), and free of clinical diseases as determined by medical history, physical examination, blood chemistries, and blood pressure and electrocardiogram at rest and during an incremental treadmill exercise. All postmenopausal women were amenorrheic≥1 year and women≤56 years of age had a follicular stimulating hormone concentration≥40 IU/L to confirm postmenopausal status. The trial was conducted in accordance with the Declaration of Helsinki and all procedures were reviewed and approved by the Institutional Review Board at the University of Colorado Boulder. The nature, risks, and benefits of all study procedures were explained to volunteers, and their written informed consent was obtained before participation in the study. This study was registered at ClinicalTrails.gov (NCT01968564).

2.2 Measurements

All measurements were performed at the University of Colorado Boulder Clinical Translational Research Center (CTRC). Participant characteristic measures, including blood assays, were performed after a > 12-hour fast from food (water allowed), caffeine, and dietary supplements, and >24-hour refrainment from alcohol, physical activity, and prescription medication. Motor and cognitive measures were obtained after a > 2-hour snack or light meal and >24-hour refrainment from alcohol [18].

2.3 Curcumin administration

In this double-blind study, participants were randomized and stratified for sex to placebo (2000 mg/day: 99.5% maltodextrin and 0.05% tartrazine, yellow food coloring) or curcumin (2000 mg/day Longvida^®: ∼400 mg curcumin, solid lipid particle formulation) supplementation for 12 weeks. Capsules were provided by Verdure Sciences (Noblesville, IN) and taken each morning while fasted. Previously published work with this formulation detected circulating curcumin after 650 mg ingestion and found that 2–4 g Longvida^® is safe and well tolerated [29].

2.4 Participant characteristics

Body mass, body mass index, and waist and hip circumferences were measured by anthropometry [30]. Lean mass and percent body fat were determined with dual-energy X-ray absorptiometry (DEXA; GE Lunar Prodigy Advance). Resting brachial artery systolic and diastolic blood pressures and heart rate were measured in triplicate with a semi-automated device (Dinamap XL, Johnson & Johnson). Maximal aerobic capacity (VO₂ max) was determined from open circuit spirometry during an incremental treadmill exercise test (Balke protocol) to exhaustion [31]. Weekly physical activity energy expenditure was assessed with the Community Healthy Activities Model Program for Seniors questionnaire [32]. All blood samples were collected from the cubital vein and assayed by the Colorado Clinical and Translational Sciences Institute CTRC Core laboratory or the Boulder Community Hospital Clinical laboratory, as previously described [19]. Briefly, fasting glucose, insulin, and serum lipids were determined by reflective spectrophotometry (Ortho Clinical Diagnostics), radioimmunoassay (Millipore), or standard assays, respectively.

2.5 Motor measurements

Motor function was assessed with select measures of the NIH Toolbox Motor Battery as previously described [33], including domains of strength (Grip Strength Test), dexterity (9-Hole Pegboard Dexterity Test), and mobility (4-Meter Walk Gait Speed Test). In addition to the NIH Toolbox, strength (knee flexion and extension torque; Chair Stand Test) and rate of torque development (knee flexion and extension), mobility (stair ascent test), endurance (6-Minute Walk Test; treadmill endurance during a Balke treadmill task), balance (rapid step test time and number of errors), and fatigability (heel-rise test; Fatigue Severity Scale questionnaire) were also evaluated with other established measures as described previously by our laboratory and others [18 , 34–39].

2.6 Cognition measurements

Cognitive function was evaluated with the NIH Toolbox-Cognition Battery (desktop version) as previously described [28, 40]. Briefly, the NIH Toolbox-Cognition Battery entails seven computerized tests that assess five major cognitive subdomains including processing speed (Pattern Comparison Processing Speed Test), executive function (Dimensional Change Card Sort Test; Flanker Inhibitory Control and Attention Test), working memory (List Sorting Working Memory Test), episodic memory (Picture Sequence Memory Test), and language (Picture Vocabulary Test; Oral Reading Recognition Test). The fluid composite cognition score is a combination of the NIH Toolbox tests assessing the subdomains of fluid cognitive ability: processing speed, executive function, working memory, and episodic memory. The crystallized cognition composite score is a combination of the tests assessing the subdomain of crystallized cognitive ability: language. The total cognitive function composite score, a measure of general cognitive function, is a combination of all tests listed under the fluid and crystallized composites. In addition to the NIH Toolbox-Cognition Battery, the Trail Making Test (TMT) parts A and B were administered to determine processing speed and executive function, respectively [41]. Fluid cognitive ability is primarily dependent on biological processes and declines with advancing age. In contrast, crystallized ability is influenced largely by knowledge acquired through the lifetime, is stable with age, and is not expected to change with curcumin but was included as part of the NIH Toolbox [27, 28].

2.7 Data analysis

Data are expressed as mean±standard error (SE). Statistical analysis was performed with IBM SPSS 23. Data normality was assessed with the Shapiro-Wilk test and non-normally distributed variables were log base ten transformed for parametric statistical analysis. Outliers (≥3 standard deviations) were removed for pairwise analysis. An independent t-test was performed to assess group differences at baseline. A mixed-model ANOVA was performed to identify group (placebo vs. curcumin) by time (week 0 vs. 12) interactions for all primary outcomes and clinical characteristics. Additionally, for all primary outcomes, age was added as a covariate to each ANOVA to determine if age modified the response to the intervention. In the case of significant interactions, a paired t-test was performed for within group comparisons with Bonferroni correction. Statistical significance was set at α< 0.05.

3 Results

3.1 Participants

Participant characteristics, exclusion, withdrawal, adherence, safety, and vascular outcomes for this study have been previously reported [10]. Overall, thirty-nine subjects were included in analysis (placebo n = 19, curcumin n = 21). Individuals were of non-Hispanic Caucasian (n = 32), non-Hispanic Asian (n = 3), Hispanic Caucasian (n = 2), non-Hispanic African American (n = 1), or non-Hispanic American Indian/Alaskan (n = 1) ethnicity and race, respectively. All participants had some level of college education, with ∼80% having a Bachelor’s or graduate degree.

3.2 Participant characteristics

All participant characteristics (age, body mass index, waist to hip ratio, lean mass, percent body fat, blood pressures, glucose, insulin, lipids, aerobic capacity, and physical activity energy expenditure) were not different between groups at baseline (all P > 0.05), except for body mass, which was higher in the placebo group (P = 0.03). No participant characteristics changed with time (all P > 0.2) or with time between groups (all P > 0.1; Table 1).

Table 1
Participant characteristics

Placebo Curcumin

Week 0 Week 12 Week 0 Week 12

N, men/women 11/8 – 10/10 –

Age, years 61±2 – 63±2 –

Body mass, kg 76±3* 75±3 68±2 68±3

Body mass index, kg/m² 25±1 25±1 24±1 24±1

Waist to hip ratio, U^L 0.81±0.05 0.85±0.02 0.84±0.02 0.84±0.02

Lean mass, kg 53±2 52±2 47±2 47±2

Body fat, % 27.9±2.0 27.8±2.0 30.1±1.9 30.2±1.9

Systolic blood pressure, mmHg 120±3 122±3 121±3 121±3

Diastolic blood pressure, mmHg 73±2 73±1 72±1 71±1

Resting heart rate, beats/min^L 56±2 55±2 55±1 57±2

Fasting glucose, mg/dL 84±2 85±2 85±2 87±2

Fasting insulin, μU/mL^L 8±1 8±1 7±1 8±1

Total cholesterol, mg/dL 177±6 173±6 175±8 174±6

High density lipoprotein-cholesterol, mg/dL^L 56±4 52±4 55±5 56±4

Low density lipoprotein-cholesterol, mg/dL 103±6 102±6 103±7 101±6

Triglycerides, mg/dL^L 97±15 93±15 86±11 91±12

VO₂ max, mL/kg/min 33±1 33±1 31±1 31±1

Physical activity energy expenditure, kcal/wk^L 6095±872 5334±819 5720±954 5416±984

	Placebo	Curcumin
N, men/women	11/8	–	10/10	–
Age, years	61±2	–	63±2	–
Body mass, kg	76±3*	75±3	68±2	68±3
Body mass index, kg/m²	25±1	25±1	24±1	24±1
Waist to hip ratio, U^L	0.81±0.05	0.85±0.02	0.84±0.02	0.84±0.02
Lean mass, kg	53±2	52±2	47±2	47±2
Body fat, %	27.9±2.0	27.8±2.0	30.1±1.9	30.2±1.9
Systolic blood pressure, mmHg	120±3	122±3	121±3	121±3
Diastolic blood pressure, mmHg	73±2	73±1	72±1	71±1
Resting heart rate, beats/min^L	56±2	55±2	55±1	57±2
Fasting glucose, mg/dL	84±2	85±2	85±2	87±2
Fasting insulin, μU/mL^L	8±1	8±1	7±1	8±1
Total cholesterol, mg/dL	177±6	173±6	175±8	174±6
High density lipoprotein-cholesterol, mg/dL^L	56±4	52±4	55±5	56±4
Low density lipoprotein-cholesterol, mg/dL	103±6	102±6	103±7	101±6
Triglycerides, mg/dL^L	97±15	93±15	86±11	91±12
VO₂ max, mL/kg/min	33±1	33±1	31±1	31±1
Physical activity energy expenditure, kcal/wk^L	6095±872	5334±819	5720±954	5416±984

Data are mean±SE; ^LData log transformed for statistical analysis; *P = 0.03 vs. curcumin week 0.

3.3 Motor measurements

All motor function outcomes are presented in Table 2. Motor function measures were not different at baseline between the placebo and curcumin groups (all P > 0.1). Additionally, there were no time (P > 0.1) or group by time interactions (P > 0.05) for measures of muscle strength (grip strength, chair stand, knee flexion and extension torque) and rate of torque development, dexterity (9-hole pegboard dexterity), mobility (4-meter walk gait speed, stair ascent), endurance (treadmill endurance), balance (rapid step test time), and fatigability (heel rise). 6-minute walk distance increased 2% (P = 0.02), the number of errors for the rapid step test was lower (P = 0.004), and scores on the Fatigue Severity Scale questionnaire were lower with time (P = 0.02), however there were no group by time interactions for these measures (all P > 0.4). The addition of age as a covariate for all motor measurements did not alter the above reported responses to the intervention.

Table 2
Measures of motor function before and after 12 weeks of placebo or curcumin supplementation

Placebo Curcumin P value

Week 0 Week 12 Week 0 Week 12 Time Group by time

Muscle strength

^NIHTBGrip strength, kg^L 32.6±1.8 33.5±2.0 29.9±2.4 30.1±2.4 0.5 0.6

Chair stand, s 9.4±0.5 8.8±0.6 8.5±0.3 8.8±0.3 0.4 0.05

Knee flexion torque, J 78.4±7.6 73.3±7.3 69.1±4.8 64.3±7.0 0.3 0.97

Knee extension torque, J^L 83.6±14.7 62.4±8.5 74.8±9.9 65.9±7.7 0.1 0.7

Muscle rate of torque development (RTD)

Knee flexion RTD, J/s 737.5±130.0 626.2±94.2 557.0±96.7 498.7±52.1 0.1 0.6

Knee extension RTD, J/s^L 554.8±93.2 481.3±60.4 582.2±88.5 548.9±77.7 0.5 0.4

Manual dexterity

^NIHTB9-hole pegboard dexterity, s 20±1 20±1 20±1 19±1 0.2 0.6

Mobility

^NIHTB4-meter walk gait speed, m/s 1.2±0.04 1.2±0.04 1.2±0.04 1.2±0.03 0.98 0.9

Stair ascent, s^L 3.5±0.2 3.5±0.2 3.5±0.1 3.6±0.1 0.4 0.6

Endurance

6-minute walk, m 606.7±17.9 616.2±19.3 585.2±14.6 600.2±11.6 0.02 0.6

Treadmill endurance, s 582±18 587±26 543±19 544±26 0.7 0.8

Balance

Rapid step test, s^L 33.7±1.0 32.8±1.0 35.1±1.0 33.9±1.0 0.2 0.9

Rapid step test error 2.3±0.4 1.8±0.4 2.4±0.3 1.4±0.2 0.004 0.4

Fatigability

Heel-rise, s 115±11 91±9 112±20 129±36 0.8 0.3

Fatigue severity scale^L 18±2 16±2 19±2 16±2 0.02 0.9

	Placebo	Curcumin	P value
Muscle strength
^NIHTBGrip strength, kg^L	32.6±1.8	33.5±2.0	29.9±2.4	30.1±2.4	0.5	0.6
Chair stand, s	9.4±0.5	8.8±0.6	8.5±0.3	8.8±0.3	0.4	0.05
Knee flexion torque, J	78.4±7.6	73.3±7.3	69.1±4.8	64.3±7.0	0.3	0.97
Knee extension torque, J^L	83.6±14.7	62.4±8.5	74.8±9.9	65.9±7.7	0.1	0.7
Muscle rate of torque development (RTD)
Knee flexion RTD, J/s	737.5±130.0	626.2±94.2	557.0±96.7	498.7±52.1	0.1	0.6
Knee extension RTD, J/s^L	554.8±93.2	481.3±60.4	582.2±88.5	548.9±77.7	0.5	0.4
Manual dexterity
^NIHTB9-hole pegboard dexterity, s	20±1	20±1	20±1	19±1	0.2	0.6
Mobility
^NIHTB4-meter walk gait speed, m/s	1.2±0.04	1.2±0.04	1.2±0.04	1.2±0.03	0.98	0.9
Stair ascent, s^L	3.5±0.2	3.5±0.2	3.5±0.1	3.6±0.1	0.4	0.6
Endurance
6-minute walk, m	606.7±17.9	616.2±19.3	585.2±14.6	600.2±11.6	0.02	0.6
Treadmill endurance, s	582±18	587±26	543±19	544±26	0.7	0.8
Balance
Rapid step test, s^L	33.7±1.0	32.8±1.0	35.1±1.0	33.9±1.0	0.2	0.9
Rapid step test error	2.3±0.4	1.8±0.4	2.4±0.3	1.4±0.2	0.004	0.4
Fatigability
Heel-rise, s	115±11	91±9	112±20	129±36	0.8	0.3
Fatigue severity scale^L	18±2	16±2	19±2	16±2	0.02	0.9

Data are mean±SE; ^NIHTBNIH Toolbox measure; ^LData log transformed for statistical analysis.

3.4 Cognitive measurements

All cognitive function outcomes are presented in Table 3. There were no baseline differences in individual tests of cognitive function between the placebo and curcumin group (all P > 0.1). There were no changes with time (all P > 0.05) or group by time interactions (all P > 0.06) for processing speed (pattern comparison processing speed, trail making test-A), executive function (dimensional change card sort, trail making test-B), working memory (list sorting working memory), and language (picture vocabulary) between the placebo and curcumin group. However, flanker inhibitory control and attention, a measure of executive function increased 2% (P = 0.04) and picture sequence memory, a measure of episodic memory increased 7% (P < 0.05) with time but there was no group by time interactions (both P > 0.3). Additionally, there was no change with time (P = 0.2) for reading decoding, but a group by time interaction (P = 0.001) was observed. Reading decoding was unchanged in the placebo group (P = 0.07) but there was a 3% increase in the curcumin group (P = 0.003) after 12 weeks.

Table 3
Measures of cognitive function before and after 12 weeks of placebo or curcumin supplementation

Placebo Curcumin P value

Week 0 Week 12 Week 0 Week 12 Time Group by time

Processing speed

^NIHTBPattern comparison processing speed 51±2 52±2 55±3 58±3 0.2 0.5

Trail making test-A, s^L 27.02±1.91 25.68±1.77 28.02±2.05 27.67±1.94 0.4 0.6

Executive function

^NIHTBDimensional change card sort 8.13±0.12 8.13±0.16 7.78±0.14 7.93±0.17 0.4 0.4

^NIHTBFlanker inhibitory control and attention 8.3±0.1 8.5±0.1 8.3±0.1 8.5±0.1 0.04 0.97

Trail making test-B, s^L 61.70±4.13 58.08±4.71 57.20±5.31 52.88±3.83 0.1 0.9

Working memory

^NIHTBList sorting working memory 17±1 17±1 17±1 17±1 0.5 0.97

Episodic memory

^NIHTBPicture sequence memory 487.4±23.6 501.7±25.3 475.1±22.2 516.8±25.6 0.05 0.3

Language

^NIHTBPicture vocabulary 1890.2±36.8 1952.0±46.9 1970.2±40.3 1968.3±45.5 0.08 0.06

^NIHTBOral reading recognition 2364±40 2334±39 2357±34 2428±35* 0.2 0.001

Cognitive function composite scores

^NIHTBFluid 101.6±1.8 103.1±2.2 101.5±2.3 104.2±2.8 0.07 0.6

^NIHTBCrystallized 129.5±2.4 130.2±2.5 131.7±2.4 133.7±2.6 0.07 0.3

^NIHTBTotal 119.7±2.6 121.3±3.4 122.9±3.4 126.8±4.1 0.03 0.3

	Placebo	Curcumin	P value
Processing speed
^NIHTBPattern comparison processing speed	51±2	52±2	55±3	58±3	0.2	0.5
Trail making test-A, s^L	27.02±1.91	25.68±1.77	28.02±2.05	27.67±1.94	0.4	0.6
Executive function
^NIHTBDimensional change card sort	8.13±0.12	8.13±0.16	7.78±0.14	7.93±0.17	0.4	0.4
^NIHTBFlanker inhibitory control and attention	8.3±0.1	8.5±0.1	8.3±0.1	8.5±0.1	0.04	0.97
Trail making test-B, s^L	61.70±4.13	58.08±4.71	57.20±5.31	52.88±3.83	0.1	0.9
Working memory
^NIHTBList sorting working memory	17±1	17±1	17±1	17±1	0.5	0.97
Episodic memory
^NIHTBPicture sequence memory	487.4±23.6	501.7±25.3	475.1±22.2	516.8±25.6	0.05	0.3
Language
^NIHTBPicture vocabulary	1890.2±36.8	1952.0±46.9	1970.2±40.3	1968.3±45.5	0.08	0.06
^NIHTBOral reading recognition	2364±40	2334±39	2357±34	2428±35*	0.2	0.001
Cognitive function composite scores
^NIHTBFluid	101.6±1.8	103.1±2.2	101.5±2.3	104.2±2.8	0.07	0.6
^NIHTBCrystallized	129.5±2.4	130.2±2.5	131.7±2.4	133.7±2.6	0.07	0.3
^NIHTBTotal	119.7±2.6	121.3±3.4	122.9±3.4	126.8±4.1	0.03	0.3

Data are mean±SE; ^NIHTBNIH Toolbox measure; ^LData log transformed for statistical analysis; *P = 0.003 vs. curcumin week 0.

There were no baseline differences in fluid, crystallized, and total cognitive function composite scores between groups (all P > 0.4). At baseline the placebo group’s age-adjusted national percentile for the fluid, crystallized, and total cognitive function composite scores were at the 68th, 87th, and 83rd percentile, respectively, where the curcumin group scored at the 65th, 92nd, and 87th percentile, respectively, with no differences between groups (all P > 0.5). Fluid and crystallized composite cognition scores slightly increased 1–2% with time (both P = 0.07) but there was no change with time between groups (both P > 0.3). Total cognitive function composite score, a combination of all tests under fluid and crystallized composites, marginally increased by 2% with time (P = 0.03) but no group by time interaction (P = 0.3) between the placebo and curcumin group was observed. The addition of age as a covariate for all cognitive measurements did not alter the above reported response to the intervention.

4 Discussion

This is the first study to assess the effects of curcumin supplementation on motor and cognitive function in healthy middle-aged and older individuals. After 12 weeks of curcumin supplementation compared with placebo, there were no changes in any subdomains of motor or cognitive function as assessed with the NIH Toolbox and other standardized tests, except for a marginal 3% increase in reading decoding ability. Overall, the present findings suggest that 12 weeks of curcumin supplementation has no apparent effect on motor or cognitive function domains that decline with age in healthy high-functioning middle-aged and older adults.

4.1 Curcumin supplementation and motor function

Motor function, including muscle strength, dexterity, fatigability, mobility, endurance, and balance, decline with advancing age [21 –26] and are predictive of current and future disability and mortality [4–6 , 42]. Identification of intervention strategies that delay the onset of age-related declines in physiological function, including motor ability, hold promise to improve independence and quality of life.

Information on the effects of curcumin supplementation on motor function in the context of aging is surprisingly limited. In animal models of chronic disease, including Parkinson’s disease, curcumin protects against disease-associated motor impairments [43, 44], whereas no studies in preclinical models of healthy aging have been performed. In humans, one investigation reported a decline in self-reported physical fatigue after 4-weeks of curcumin supplementation in healthy older adults [45], however no other studies have assessed the impact of curcumin supplementation on multiple subdomains of motor performance in middle-aged and older adults without evident motor impairment. In the present trial, we found no evidence of improvement in any subdomain of motor function after 12 weeks of curcumin supplementation. A minor time effect was observed for some motor function measures (rapid step error, fatigue severity scale, and six-minute walk) after the 12-week intervention. It is difficult to discern if this was a slight practice effect or due to a type 1 error. Regardless, there was no evidence in our study that 12 weeks of curcumin supplementation influenced motor function in healthy middle-aged and older adults.

4.2 Curcumin supplementation and cognitive function

Advancing age is associated with a decline in cognitive function, which primarily manifests as reduced fluid ability–the ability to solve novel problems and encode episodic memories (27, 28]. Maintaining optimal levels of cognitive function during aging would contribute importantly to preservation of healthspan.

In animal models of chronic disease, primarily Alzheimer’s disease, curcumin attenuates disease-associated cognitive impairments [46 –49]. Furthermore, curcumin improves cognitive function, including learning and memory, in rodent models of accelerated aging [50, 51] as well as healthy older rodents [52 –54]. Findings regarding the effect of curcumin on cognitive function in older humans have been inconsistent. Cox et al. reported that in participants who were on average older than those in the current study (60–85 vs. 45–74 yrs), 4 weeks of curcumin supplementation improved working memory and attention [45]. Another investigation in middle-aged and older adults, including individuals with and without risk factors for cardiovascular disease (e.g., hypertension), found that 12 months of curcumin supplementation had no effect on multiple subdomains of cognitive function [55]. In contrast to previous investigations, the current trial was conducted in entirely healthy individuals and included both middle-aged and older adults. In these participants, 12 weeks of curcumin supplementation did not influence any subdomains of cognitive function that decline with age. Although there was a minor 3% increase in reading decoding ability, these data are difficult to interpret because this measure of crystallized ability remains stable with aging and, as such, was not expected to change with intervention.

Although in the present study we used the same curcumin formulation as the study by Cox et al. [45], even a higher dose (2000 vs. 400 mg/day Longvida^®), differences between our investigation and that by Cox et al. may be due to a number of different factors, including the tests used to measure working memory or participant age. The subjects in the study by Cox et al. were all over the age of 60 (60–85 yrs) whereas individuals in the current trial were on average younger (45–74 yrs). Additionally, Cox et al. cited age-related differences in cognitive function between their subjects and young individuals, but baseline age percentiles for fluid and relative cognitive ability of the participants were not reported. As such, it is possible that subjects in the current study had higher baseline cognitive function and less potential for improvement with curcumin supplementation (“ceiling effect”), as discussed next.

4.3 Relation with vascular endothelial function and potential influence of baseline function

We have previously reported that 12 weeks of curcumin supplementation improved vascular endothelial function by 35–40% in the participants of the present investigation. Vascular endothelial function has been suggested to be a determinant of motor and cognitive function in older adults [15 , 56], and there are examples of other nutraceuticals improving vascular, motor, and cognitive function in healthy middle-aged and older adults [18, 19]. In contrast, despite improving vascular endothelial function by reducing oxidative stress and increasing vascular nitric oxide bioavailability [10], we found no significant group changes in motor or cognitive function with curcumin supplementation in the present analysis. To further explore potential links between changes in vascular endothelial function and cognitive and/or motor function with curcumin supplementation in our cohort, we assessed the relation between improvements in cognitive and motor function (present analysis) and brachial artery flow-mediated dilation from our previously published data [10]. We found no significant correlations between individual changes in vascular endothelial function and cognitive or motor functions in response to 12 weeks of curcumin supplementation. Thus, although vascular function, oxidative stress, and nitric oxide bioavailability influence systemic processes, improvements in these biological properties did not translate to changes in motor or cognitive functions in the present group of healthy middle-aged and older adults. It is possible that these functions are not strongly related in healthy adults, or that the magnitude and/or duration (12-week intervention) of the improvements were not sufficient to evoke corresponding improvements in motor or cognitive performance this group.

Concerning the first possible explanation, subjects in this curcumin supplementation study were recruited based on the presence of impaired baseline vascular endothelial function, as this was a primary outcome. In contrast, participants were within the normal expected age ranges for motor and function, including gait speed [57], endurance [58], and strength [59]. Our subjects were not only free from baseline cognitive dysfunction (e.g., mild cognitive impairments or dementia), but had some form of higher education, and were well above the national age percentile for cognitive function at baseline (the curcumin group scored at the 65th and 92nd percentile for fluid and crystallized composite scores, respectively). Thus, any potential improvements in cognitive function would likely be difficult to detect given the high initial cognitive abilities of our participants. Although curcumin had no effect on motor or cognitive function in this healthy population, future studies are needed to determine if curcumin may improve motor or cognitive function in individuals with lower baseline motor and cognitive function or clinical impairments in these functional domains.

4.4 Short-term intervention and NIH Toolbox cognitive battery

The NIH Toolbox motor and cognitive batteries are recently released standardized batteries that are part of the NIH Toolbox for the Assessment of Neurological and Behavioral Function specifically developed for use in intervention studies and clinical trials. These validated, time-efficient batteries allow for the comprehensive assessment of multiple domains of motor and cognitive function, enabling standardized measurements and age comparisons across studies. Due to its recent availability, all previous reports in healthy adults have been cross-sectional comparisons [33 , 60–62] or re-testing performed after 2 weeks for validation [40 , 63–66]. To our knowledge, this is the first investigation to utilize the NIH Toolbox motor and cognitive batteries in an intervention study. It is important to acknowledge that many of the measures of the NIH Toolbox cognitive battery demonstrated a time effect during re-testing, 12 weeks after baseline. Previous studies when re-testing has been performed after 2 weeks have provided evidence of practice effects for the cognitive battery [62 –64]. We are unable to discern if the slight improvements in the cognitive scores with time observed in the current trial are due to true learning effects or a result of a drift in testing scores, as improvements were minor, ranging from 1 to 7%. Regardless, our placebo-controlled experimental design allowed us to evaluate the potential independent effects of curcumin, and we did not document any additive influence of curcumin in cognitive tests where learning effects were observed. However, more studies are needed to determine if longer intervention times are needed to avoid the observed time effects when using the NIH Toolbox cognitive battery.

4.5 Limitations

This study was primarily in Caucasian men and women; therefore, it is unknown if curcumin supplementation improves motor or cognitive function in healthy middle-aged and older adults of other ethnicities. Additionally, as discussed above, the participants in the present trial were mainly college educated and had high cognitive function, and were also free from motor impairments. Individuals with chronic diseases or clinically significant impairments were not included in this investigation to initially evaluate the effects of curcumin on healthy aging.

4.6 Conclusions

In healthy, well-educated middle-aged and older adults without motor or cognitive impairments and high age-adjusted baseline performance, 12 weeks of curcumin supplementation did not influence motor or cognitive function domains that decline with age. These data support the view that adding curcumin supplements to the normal diet will likely not further enhance motor or cognitive performance in that subgroup of the aging population. However, additional studies are needed to determine if curcumin supplementation has beneficial effects in individuals or groups with more significantly reduced baseline motor and cognitive function as a result of older age and/or clinical disorders.

Footnotes

Acknowledgments

The authors thank Jonathan J. Herrera, Chasen W. Daugherty, and the staff of the University of Colorado Boulder Clinical and Translational Research Center for their technical assistance. J.N.J. is currently at Internal Medicine – Section on Gerontology and Geriatrics at Wake Forest School of Medicine, Winston-Salem, NC (current affiliated email: jnjustic@wakehealth.edu). This work was supported by National Institutes of Health awards R21 AG042795, Colorado CTSA UL1 TR001082, T32 AG000279-14S1, R37 AG013038-17S1, and R21 AG042795-01A1S1. Contents are the authors’ sole responsibility and do not necessarily represent official National Institutes of Health views. The authors have no declarations to disclose.

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	Placebo		Curcumin
	Week 0	Week 12	Week 0	Week 12
N, men/women	11/8	–	10/10	–
Age, years	61±2	–	63±2	–
Body mass, kg	76±3*	75±3	68±2	68±3
Body mass index, kg/m²	25±1	25±1	24±1	24±1
Waist to hip ratio, U^L	0.81±0.05	0.85±0.02	0.84±0.02	0.84±0.02
Lean mass, kg	53±2	52±2	47±2	47±2
Body fat, %	27.9±2.0	27.8±2.0	30.1±1.9	30.2±1.9
Systolic blood pressure, mmHg	120±3	122±3	121±3	121±3
Diastolic blood pressure, mmHg	73±2	73±1	72±1	71±1
Resting heart rate, beats/min^L	56±2	55±2	55±1	57±2
Fasting glucose, mg/dL	84±2	85±2	85±2	87±2
Fasting insulin, μU/mL^L	8±1	8±1	7±1	8±1
Total cholesterol, mg/dL	177±6	173±6	175±8	174±6
High density lipoprotein-cholesterol, mg/dL^L	56±4	52±4	55±5	56±4
Low density lipoprotein-cholesterol, mg/dL	103±6	102±6	103±7	101±6
Triglycerides, mg/dL^L	97±15	93±15	86±11	91±12
VO₂ max, mL/kg/min	33±1	33±1	31±1	31±1
Physical activity energy expenditure, kcal/wk^L	6095±872	5334±819	5720±954	5416±984