Restricted accessResearch articleFirst published online 2022-09-15
Caffeine citrate effects on gastrointestinal permeability,bacterial translocation and biochemical parameters in newborn rats after long-term oral administration
Caffeine is a potent central and respiratory acting agent used in neonatology to treat apnea in premature newborns.
OBJECTIVE:
This study investigates the effects of caffeine orally administered to newborn rats on gastrointestinal permeability, bacterial translocation and different biochemical parameters.
METHODS:
Newborn rats were divided into different groups (N = 06). The treated newborn rats were orally administered with standard caffeine doses (12 mg/kg per day), and the control groups received a placebo. The animals were weighed daily until sacrifice. Blood samples, mesenteric lymph nodes (MLN) and organs were aseptically collected. Furthermore, different biochemical (D-Lactate) and oxidative stress biomarkers (MDA, CAT, SOD and GSH) were examined. Microbiological analyses were performed to assess microbiota alterations and bacterial translocation.
RESULTS:
Preliminary results showed that caffeine administration decreased the level of bacterial translocation over time. The treatment reduced plasma D-lactate levels (p < 0.05). Additionally, caffeine induced a disturbance in the concentrations of biochemical parameters and oxidative stress biomarkers. Indeed, liver enzymes (AST and ALT) were significantly (p < 0.05) risen after caffeine treatment. Glutathione (GSH) levels were significantly higher in caffeine treated groups (75.12±0.32; 51.98±1.12 U/mg; p < 0.05) comparing to control ones (40.82±0.25; 42.91±0.27 U/mg; p < 0.05) in the ileum and the colon, respectively.
CONCLUSIONS:
Thus, besides improving gastrointestinal permeability, our data show that caffeine has beneficial effects on the intestinal antioxidant system.
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