GC-MS analysis of chemical composition,cytotoxicity and antioxidant activities of essential oils of Senecio glaucus under drastic conditions

Abstract

This study was designed to analyze essential oils by GC-MS and to evaluate the chemical composition, cytotoxic and antioxidant activity of aerial part of plant Seniso glaucus using two methods hydro distillation and organic solvent extraction. The essential oil was analyzed by GC-MS revealed the presence of 32 compounds most of them are sesquiterpene, the main component of essential oil were, Isolongifolene, 9-one (14.4%) Longiverbenone (13.5%), (+) 4-Carene (8.39 %), O-cymene, 4.64%, and thujone were 3.54%. Cytotoxic activity of essential oil was tested against four carcinoma cell lines (A549, McF7, HepG, and HCT-16) by using (3- (4, 5- dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide) (MTT), the results indicate promising cytotoxic activity at the concentration (100, and50μg/ml) essential oil exhibit high cytotoxic activities against Hepg2 cell lines (83. 33 and 59.14 %) and good cytotoxicity against A549, McF-7 and HCT116 cell lines by (62.1 %, 74.40 %, and 76.60%respectively). The essential oil showed antioxidant activity with IC₅₀ = 69.59±0.24μg/ml, using 2, 2-diphenyl-1- picrylhydrazyl (DPPH) assay the present study concluded the importance of plant Seniso glaucus as a natural antioxidant.