In reply to the increasing request of structural and molecular dynamics data taken on biological samples at both high temperature and high pressure by neutron scattering techniques, we have developed a new generation of high pressure cells with the following characteristics: (1) a flat geometry to better exploit the neutron beam’s cross section, (2) utilisable in the pressure range between 0 and 600 MPa and up to 100°C, (3) adapted for studies by coherent and incoherent neutron scattering, and (4) compatible with all types of neutron diffractometers and spectrometers at the Institut Laue-Langevin in Grenoble, France.
Such high pressure cells permit to explore domains of pressure and temperature inaccessible so far, to establish diagrams and determine phase transitions. They are particularly adapted to the investigation of biological systems living under extreme conditions, as for instance extremophiles living near hot vents in the deep sea, or the response of biomolecules to extreme loads.
B.Aoun, E.Pellegrini, M.Trapp, F.Natali, L.Cantù, P.Brocca, Y.Gerelli, B.Demé, M.M.Koza, M.Johnson and J.Peters, Direct comparison of elastic incoherent neutron scattering experiments with molecular dynamics simulations of DMPC phase transitions, The European Physical Journal E39(4) (2016), 48. doi:10.1140/epje/i2016-16048-y.
3.
R.Bosco, J.V.D.Beucken, S.Leeuwenburgh and J.Jansen, Surface engineering for bone implants: A trend from passive to active surfaces, Coatings2(3) (2012), 95–119, ISSN 2079-6412. doi:10.3390/coatings2030095.
4.
R.E.Collins, G.Rocap and J.W.Deming, Persistence of bacterial and archaeal communities in sea ice through an Arctic winter, Environmental Microbiology12(7) (2010), 1828–1841, ISSN 1462–2920. doi:10.1111/j.1462-2920.2010.02179.x.
5.
V.Cristiglio, B.Giroud, L.Didier and B.Demé, D16 is back to business: More neutrons, more space, more fun, Neutron News26(3) (2015), 22–24. doi:10.1080/10448632.2015.1057051.
6.
B.Deschaud and J.-M.Muiras, Essais de traction et duretés, Technical Report, CET0137626, CETIM, 7 rue de la Presse, CS 50802, 42952 Saint-Etienne Cedex 1, France, 2017.
M.Jebbar, B.Franzetti, E.Girard and P.Oger, Microbial diversity and adaptation to high hydrostatic pressure in deep-sea hydrothermal vents prokaryotes, Extremophiles19 (2015), 721. doi:10.1007/s00792-015-0760-3.
9.
C.Jeworrek, R.Steitz, C.Czeslik and R.Winter, High pressure cell for neutron reflectivity measurements up to 2500 bar, Review of Scientific Instruments82(2) (2011), 025106. doi:10.1063/1.3553392.
10.
S.Klotz, High tensile aluminium alloys, in: Techniques in High Pressure Neutron Scattering, T.Francis, ed., CRC Press, Boca Raton, 2013.
11.
J.Koo and C.Czeslik, High pressure sample cell for total internal reflection fluorescence spectroscopy at pressures up to 2500 bar, Review of Scientific Instruments83(8) (2012), 085109. doi:10.1063/1.4746385.
12.
E.Lelièvre-Berna, B.Demé, J.Gonthier, J.-P.Gonzales, J.Maurice, Y.Memphis, C.Payre, P.Oger, J.Peters and S.Vial, 700 MPa sample stick for studying liquid samples or solid-gas reactions down to 1.8 K and up to 550 K, Journal of Neutron Research19(1–2) (2017), 77–84. doi:10.3233/JNR-170044.
13.
B.J.C.Luthringer, U.M.R.Katha and R.Willumeit, Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis, Journal of Materials Science: Materials in Medicine25(11) (2014), 2561–2571, ISSN 1573-4838. doi:10.1007/s10856-014-5263-8.
14.
W.Martin, J.Baross, D.Kelley and M.J.Russell, Hydrothermal vents and the origin of life, Extremophiles6 (2008), 805–814. doi:10.1038/nrmicro1991.
15.
N.Martinez, G.Michoud, A.Cario, J.Ollivier, B.Franzetti, M.Jebbar, P.Oger and J.Peters, High protein flexibility and reduced hydration water dynamics are key pressure adaptive strategies in prokaryotes, Scientific Reports6 (2016), 32816. doi:10.1038/srep32816.
16.
F.Meersman, I.Daniel, D.H.Bartlett, R.Winter, R.Hazael and P.R.McMillan, High-pressure biochemistry and biophysics, Reviews in Mineralogy and Geochemistry75 (2013), 607–648. doi:10.2138/rmg.2013.75.19.
17.
K.C.Morrell, W.A.Hodge, D.E.Krebs and R.W.Mann, Corroboration of in vivo cartilage pressures with implications for synovial joint tribology and osteoarthritis causation, Proceedings of the National Academy of Sciences102(41) (2005), 14819–14824, ISSN 0027-8424. doi:10.1073/pnas.0507117102.
18.
F.Natali, J.Peters, D.Russo, S.Barbieri, C.Chiapponi, A.Cupane, A.Deriu, M.T.D.Bari, E.Farhi, Y.Gerelli, P.Mariani, A.Paciaroni, C.Rivasseau, G.Schirò and F.Sonvico, IN13 backscattering spectrometer at ill: Looking for motions in biological macromolecules and organisms, Neutron News19(4) (2008), 14–18. doi:10.1080/10448630802474083.
19.
G.Pabst, J.Katsaras, V.A.Raghunathan and M.Rappolt, Structure and interactions in the anomalous swelling regime of phospholipid bilayers, Langmuir19(5) (2003), 1716–1722. doi:10.1021/la026052e.
20.
J.Peters, N.Martinez, G.Michoud, A.Cario, B.Franzetti, P.Oger and M.Jebbar, Deep sea microbes probed by incoherent neutron scattering under high hydrostatic pressure, Zeitschrift für Physikalische Chemie228(10–12) (2016), 1121–1133. doi:10.1515/zpch-2014-0547.
21.
J.Peters, M.Trapp, D.Hughes, S.Rowe, B.Demé, J.-L.Laborier, C.Payre, J.-P.Gonzales, S.Baudoin, N.Belkhier and E.Lelièvre-Berna, High hydrostatic pressure equipment for neutron scattering studies of samples in solutions, High Pressure Research32(1) (2012), 97–102. doi:10.1080/08957959.2011.642376.
22.
M.Rappolt and G.Pabst, Flexibility and structure of fluid bilayer interfaces, in: Structure and Dynamics of Membranous Interfaces, K.Nag, ed., John Wiley & Sons, Hoboken, NJ, USA, 2008. doi:10.1002/9780470388495.
S.S.Sidhu, L.Heaton, D.D.Zauberis and F.P.Campos, Neutron diffraction study of Titanium–Zirconium system, Journal of Applied Physics27(9) (1956), 1040–1042. doi:10.1063/1.1722538.
25.
G.N.Somero, Adaptations to high hydrostatic pressure, Annual Review of Physiology54(1) (1992), 557–577. doi:10.1146/annurev.ph.54.030192.003013.
26.
K.Takai, K.Nakamura, T.Toki, U.Tsunogai, M.Miyazaki, J.Miyazaki, H.Hirayama, S.Nakagawa, T.Nunoura and K.Horikoshi, Cell proliferation at 122°C and isotopically heavy CH4 production by a hyperthermophilic methanogen under high-pressure cultivation, Proceedings of the National Academy of Sciences105(31) (2008), 10949–10954. doi:10.1073/pnas.0712334105.
A.-M.Trunfio-Sfarghiu, Y.Berthier, M.-H.Meurisse and J.-P.Rieu, Role of nanomechanical properties in the tribological performance of phospholipid biomimetic surfaces, Langmuir24(16) (2008), 8765–8771. doi:10.1021/la8005234.
29.
R.Winter and C.Czeslik, Pressure effects on the structure of lyotropic lipid mesophases and model biomembrane systems, Zeitschrift für Kristallographie – Crystalline Materials215(8) (2009), 454–474. doi:10.1524/zkri.2000.215.8.454.
30.
R.Winter and C.Jeworrek, Effect of pressure on membranes, Soft Matter5 (2009), 3157–3173. doi:10.1039/B901690B.
31.
P.T.T.Wong and H.H.Mantsch, Effects of hydrostatic pressure on the molecular structure and endothermic phase transitions of phosphatidylcholine bilayers: A Raman scattering study, Biochemistry24(15) (1985), 4091–4096. doi:10.1021/bi00336a043.
32.
A.A.Yayanos, Evolutional and ecological implications of the properties of deep-sea barophilic bacteria, Proceedings of the National Academy of Sciences83(24) (1986), 9542–9546. doi:10.1073/pnas.83.24.9542.
33.
F.Zhu, E.Tajkhorshid and K.Schulten, Pressure-induced water transport in membrane channels studied by molecular dynamics, Biophysical Journal83(1) (2002), 154–160. doi:10.1016/S0006-3495(02)75157-6.
