Black currants are berries that attract attention due to the beneficial effects they possess. They are proven to be good antioxidants, anticancerogenic, cardio- and neuroprotective agents.
OBJECTIVE:
The main objective of this research was to evaluate the differences in the flavonols composition, antioxidant and spasmolytic activity of juices obtained from four different black currant cultivars – Triton, Tenah, Ben Sarek, and Ometa, grown over a 4-year long period on the territory of Serbia.
METHODS:
The qualitative and quantitative determination of black currants juices flavonols was performed using the HPLC. The antioxidative activity was carried out in DPPH and β-carotene systems. The study examined the impact of the main black currants flavonols and black currants juices on the spontaneous, KCl, and acetylcholine-induced ileum smooth muscle contractions.
RESULTS:
The dominant flavonol in juices of the four different black currant cultivars was myricetin, with its highest content obtained from the 2015 Triton juice (820.1±34.7 μg g–1). Quercetin was the second in abundance and the lowest yields were observed in kaempferol.
CONCLUSION:
The investigated black currant cultivars were proven to be good sources of flavonols and potential antioxidants with the significant spasmolytic activity that could be successfully used in treating gastrointestinal disorders.
BarneyDL, HummerEK. Currants, Gooseberries, and Jostaberries: Guide for Growers,Marketers, and Researchers in North America. Binghamton. NY: Food Products Press, An Imprint of The Haworth Press, Inc; 2005.
2.
Mikulic-PetkovsekM, KoronD, VebericR. Quality parameters of currant berries from three different cluster positions. Sci Hortic. 2016;210:188–96. doi: 10.1016/j.scienta.2016.07.030
3.
LutzCA, MazurEE, LitchNA. Nutrition and diet therapy, 6th ed. Philadelphia, PA: F.A. Davis Company; 2015.
4.
HeinonenM. Antioxidant activity and antimicrobial effect of berry phenolics – a Finnish perspective. Mol Nutr Food Res. 2007;51(6):684–91. doi: 10.1002/mnfr.200700006
5.
KangNJ, JungSK, LeeKW, LeeHJ. Myricetin is a potent chemopreventive phytochemical in skin carcinogenesis. Ann NY Acad Sci. 2011;1229:124–32. doi: 10.1111/j.1749-6632.2011.06122.x
6.
Gonzalez-GallegoJ, Garcia-MediavillaMV, Sanchez-CamposS, TunonMJ, In: Watson R, Preedy VR, Zibad S (eds) Polyphenols in Human Health and Disease vol. 2. London, UK: Elsevier; 2014.
7.
TörrönenR, SarkkinenE, TapolaN, HautaniemiE, KilpiK, NiskanenL. Berries modify the postprandial plasma glucose response to sucrose in healthy subjects. Brit J Nutr. 2010;103:1094–7. doi: 10.1017/S0007114509992868
8.
RendeiroC, GuerreiroJD, WilliamsCM, SpencerJP. Flavonoids as modulators of memory and learning: molecular interactions resulting in behavioral effects. P Nutr Soc. 2012;71(2):246–62. doi: 10.1017/S0029665112000146
9.
BaggaS, StraneyD. Modulation of cAMP and phosphodiesterase activity by flavonoids which induce spore germination of Nectria haematococca MP VI (Fusarium solani). Physiol Mol Plant Path. 2000;56(2):51–61.
10.
GouldKS, ListerC. Flavonoid Functions in plants. In AndersenØM, MarkhamKR. (eds.), Flavonoids. CRC Press, Boca Raton, 2006, pp. 397–442.
11.
IwashinaT. Flavonoid function and activity to plants and other organisms. Biol Sci Space. 2003;17(1):24–44.
12.
LiuM, LiX, LiuY, CaoB. Regulation of flavanone 3-hydroxylase gene involved in the flavonoid biosynthesis pathway in response to UV-B radiation and drought stress in the desert plant, Reaumuria soongorica. Plant Physiol Bioch. 2013;73:161–7. doi: 10.1016/j.plaphy.2013.09.016
13.
HeideOM, SønstebyA. Climate-photothermographs, a Tool for Ecophysiological Assessment of Effects of ClimateWarming in Crop Plants: Examples with Three Berry Crops. J Berry Research. 2020:411-8. DOI:10.3233/JBR-190490
14.
LiY, ZhangT, ChenGY. Flavonoids and Colorectal Cancer Prevention. Antioxidants (Basel). 2018;7(12):187. doi:10.3390/antiox7120187
HakkinenSH, KarenlampiSO, HeinonenIM, MykkanenHM, TorronenAR. Content of the flavonols quercetin, myricetin, and kaempferol in 25 edible berries. J Agr Food Chem. 1999;47(6):2274–9. doi: 10.1021/jf9811065
17.
OngKC, KhooCE. Biological effects of myricetin. Gen Pharmacol. 1997;29(2):121–6. Doi: 10.1016/s0306-3623(96)00421-1
18.
LiY, DingY. Minireview: Therapeutic potential of myricetin in diabetes mellitus. Food Science and Human Wellness. 2012;1(1):19–25. doi: 10.1016/j.fshw.2012.08.002
19.
KandasamyN, AshokkumarN. Renoprotective effects of myricetin restrains dyslipidemia and renal mesangial cell proliferation by the suppression of sterol regulatory element binding proteins in an experimental model of diabetic nephropathy. Eur J Pharmacol. 2014;743:53–62. doi: 10.1016/j.ejphar.2014.09.014
20.
ShimmyoY, KiharaT, AkaikeA, NiidomeT, SugimotoH. Three distinct neuroprotective functions of myricetin against glutamate-induced neuronal cell death: involvement of direct inhibition of caspase-3. J Neurosci Res. 2008;86(8):1836–45. doi: 10.1002/jnr.21629
21.
YingX, ChenX, FengY, XuHZ, ChenH, YuK, et al.Myricetin enhances osteogenic differentiation through the activation of canonical Wnt/β-catenin signaling in human bone marrow stromal cells. Eur J Pharmacol. 2014;738:22–30. doi: 10.1016/j.ejphar.2014.04.049
22.
LiuH, GuoX, ChuY, LuS. Heart protective effects and mechanism of quercetin preconditioning on anti-myocardial ischemia reperfusion (IR) injuries in rats. Gene. 2014;545(1):149–55. doi: 10.1016/j.gene.2014.04.043
23.
MohammadiHS, GoudarziI, LashkarboloukiT, AbrariK, Elahdadi SalmaniM. Chronic administration of quercetin prevents spatial learning and memory deficits provoked by chronic stress in rats. Behav Brain Res. 2014;270:196–205. doi: 10.1016/j.bbr.2014.05.015
24.
GiampieriF, AfrinS, Forbes-HernandezTY, GasparriniM, CianciosiD, Reboredo-RodriguezP, Varela-LopezA, QuilesJL, BattinoM. Autophagy in Human Health and Disease: Novel Therapeutic Opportunities. Antioxidants & Redox Signaling. 2019;30(4):577–634. http://doi.org/10.1089/ars.2017.7234DOI:10.1089/ars.2017.7234
25.
CimangaRK, MukenyiPNK, KambuOK, TonaGL, ApersS, TottéJ, PietersL, VlietinckAJ. The spasmolytic activity of extracts and some isolated compounds from the leaves of Morinda morindoides (Baker) Milne-Redh. (Rubiaceae). J Ethnopharmacol. 2010;127(2):215–20. https://doi.org/10.1016/j.jep.2009.11.018.
26.
LozoyaX, MeckesM, Abou-ZaidM, TortorielloJ, NozzolilloC, ArnasonJT. Quercetin glycosides in Psidium guajava L. leaves and determination of a spasmolytic principle. Archives of Medical Research. 1994;25(1):11–5.
27.
RevueltaM, CantabranaB, HidalgoA. Depolarization-dependent effect of flavonoids in rat uterine smooth muscle contraction elicited by CaCl2. Gen Pharmacol. 1997;29:847–57. doi:10.1016/S0306-3623(97)00002-5
28.
GilaniA, KhanA, GhayurM, AliS, HerzigJ. Antispasmodic effects of Rooibos tea (Aspalathus linearis) is mediated predominantly through K+-channel activation. Basic Clin Pharmacol Toxicol. 2006;99(5):365–73. doi: 10.1111/j.1742-7843.2006.pto_507.x
29.
El–NekeetyAA, Abdel–AzeimSH, HassanAM, HassanNS, AlySE, Abdel–WahhabMA. Quercetin inhibits the cytotoxicity and oxidative stress in liver of rats fed aflatoxin-contaminated diet. Toxicology Report. 2014;1:319–29. doi: 10.1016/j.toxrep.2014.05.014
30.
LinSY, WangYY, ChenWY, ChuangYH, PanPH, ChenCJ. Beneficial effect of quercetin on cholestatic liver injury. J Nutr Biochem. 2014;25(11):1183–95. doi: 10.1016/j.jnutbio.2014.06.003
31.
ParkHH, LeeS, SonHY, ParkSB, KimMS, ChoiEJ, et al.Flavonoids inhibit histamine release and expression of proinflammatory cytokines in mast cells. Arch Pharm Res. 2008;31(10):1303–11. doi: 10.1007/s12272-001-2110-5
32.
Konić-RistićA, ŠavikinK, ZdunićG, JankovićT, JuranićZ, MenkovićN, et al.Biological activity and chemical composition of different berry juices. Food Chem. 2011;125(4):1412–7. doi: 10.1016/j.foodchem.2010.10.018
33.
MiladinovicB, KosticM, ŠavikinK, DjordjevicB, Mihajilov-KrstevT, ZivanovicS, et al.Chemical profile and antioxidative and antimicrobial activity of juices and extracts of 4 black currants varieties (Ribes nigrum L. ). J Food Sci. 2014;79:C301–9. doi: 10.1111/1750-3841.12364
34.
KolevaII, Van BeekTA, LinssenJPH, GrootA, EvstatievaLN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Analysis. 2002;13(1):8–17. doi: 10.1002/pca.611
35.
TabartJ, KeversC, EversD, DommesJ. Ascorbic acid, phenolic acid, flavonoid and carotenoid profiles of selected extracts from Ribes nigrum. J Agr Food Chem. 2011;59(9):4763–70. doi: 10.1021/jf104445c
36.
TabartJ, KeversC, PincemailJ, DefraigneJO, DommesJ. Antioxidant capacity of black currant varies with organ, season, and cultivar. J Agr Food Chem. 2006;54(17):6271–6. doi: 10.1021/jf061112y
37.
NourV, TrandafirI, CosmulescuS. Antioxidant capacity, phenolic compounds and minerals content of blackcurrant (Ribes nigrum L. ) leaves as influenced by harvesting date and extraction method. Ind Crop Prod. 2014;53:133–9. doi: 10.1016/j.indcrop.2013.12.022
38.
Del RioD, Rodriguez–MateosA, SpencerJP, TognoliniM, BorgesG, CrozierA. Dietary (poly)phenolics in human health: structures, bioavailability, and evidence of protective effects against chronic diseases. Antioxid Redox Sign. 2013;18(14):1818–92. doi: 10.1089/ars.2012.4581
39.
ZhengJ, YangB, RuusunenV, LaaksonenO, TahvonenR, HellstenJ, et al.Compositional differences of phenolic compounds between black currant (Ribes nigrum L. ) cultivars and their response to latitude and weather conditions. J Agr Food Chem. 2012;60(26):6581–93. doi: 10.1021/jf3012739
40.
MikkonenTP, MäättäKR, HukkanenAT, KokkoHI, TörrönenAR, KärenlampiSO, et al.Flavonol content varies among black currant cultivars. J Agr Food Chem. 2001;49(7):3274–7. doi: 10.1021/jf0010228
41.
LaaksonenO, MakilaL, TahvonenR, KallioH, YangB. Sensory quality and compositional characteristics of blackcurrant juices produced by different processes. Food Chem. 2013;138(4):2421–9. doi: 10.1016/j.foodchem.2012.12.035
42.
Orjuela-PalacioJM, ZamoraMC, LanariMC. Physicochemical and dynamic sensory characterization of a yerba mate/blackcurrant instant beverage powder rich in natural antioxidants. J Berry Research. 2019:195-208. DOI: 10.3233/JBR-180342
43.
MinB, AhnDU. Mechanism of lipid peroxidation in meat and meat products A–review. Food Sci Biotechnol. 2005;14(1):152–63.
44.
JiaN, KongB, LiuQ, DiaoX, XiaX. Antioxidant activity of black currant (Ribes nigrum L. ) extract and its inhibitory effect on lipid and protein oxidation of pork patties during chilled storage. Meat Sci. 2012;91:533–9. doi: 10.1016/j.meatsci.2012.03.010
45.
JiaN, XiongY, KongB, LiuQ, XiaX. Radical scavenging activity of black currant (Ribes nigrum L. ) extract and its inhibitory effect on gastric cancer cell proliferation via induction of apoptosis. J Funct Foods. 2012;4(1):382–90. doi: 10.1016/j.jff.2012.01.009
46.
CacaceEJ, MazzaG. Extraction of anthocyanins and other phenolics from black currants with sulfured water. J Agr Food Chem. 2002;50(21):5939–46. doi: 10.1021/jf025614x
47.
DeviRC, SimSM, IsmailR. Spasmolytic effect of citral and extracts of Cymbopogon citratus on isolated rabbit ileum. J Smooth Muscle Res. 2011;47(5):143–56. DOI: 10.1540/jsmr.47.143
48.
PenuelasA, TashimaK, TsuchiyaS, MatsumotoK, NakamuraT, HorieS, YanoS. Contractile effect of TRPA1 receptor agonists in the isolated mouse intestine. Eur J Pharmacol. 2007;576(1-3):143–50. DOI:10.1016/j.ejphar.2007.08.015
49.
OmarE, PavlovicI, DrobacM, RadenkovicM, BrankovicS, KovacevicN. Chemical composition and spasmolytic activity of Cymbopogon nervatus (Hochst. ) Chiov. (Poaceae) essential oil. Ind Crops Prod. 2016;91:249–54. https://doi.org/10.1016/j.indcrop.2016.07.013
50.
MiladinovicB, BrankovicS, KosticM, MilutinovicM, KiticN, ŠavikinK, KitićD. Antispasmodic effect of black currant (Ribes nigrumL. ) juice and its potential use as functional food in gastrointestinal disorders. Med Princ Pract. 2018;27:179–85. doi:10.1159/000487202
51.
MilutinovićM, BrankovićS, ŠavikinK, KostićM, KitićN, MiladinovićB, et al. Antispasmodic effects of black chokeberry (Aronia melanocarpa (Michx.) Elliott) extracts and juice and their potential use in gastrointestinal disorders. J Berry Res. 2019;1-18. DOI:10.3233/JBR-190435
GilaniA, KhanA, RaoofM, GhayuorM, SiddiquiB, VohraW, et al.Gastrointestinal, selective airways and urinary bladder relaxant effect of Hyoscyamus nigerare mediated through dual blockade of muscarinic receptors and Ca2+channels. Fundam Clin Pharmacol. 2008;22:87–99. doi:10.1111/j.1472-8206.2007.00561.x
54.
MoralesMA, TortorielloJ, MeckesM, et al.Calcium-antagonist effect of quercetin and its relation with the spasmolytic properties of Psidium guajava L. Arch Med Res. 1994;25:17–21.
55.
BrankovicS, KiticD, RadenkovicM, VeljkovicS, GolubovicT. Calcium blocking activity as a mechanism of the spasmolytic effect the essential oil of Calamintha glandulosa Silic on isolated rat ileum. Gen Physiol Biophs. 2009;28:174–8.
56.
GuataY, AminataS, AmadouM, BabacarF. Myorelaxant and antispasmodic effects of the aqueous extract of Mitragyna inermis barks on Wistar rat ileum. Fitoterapia. 2004;75(5):447–50. doi: 10.1016/j.fitote.2004.03.006
