Restricted accessResearch articleFirst published online 2019-08-28
Effectiveness of different depuration procedures in removing reagents interference on in vitro digested strawberry extracts for reliable antioxidant determinations
Healthy benefits associated with strawberries consumption are mostly related to their antioxidant composition, mainly polyphenols. Quality assessment on fresh fruits is commonly done by spectrophotometric methods, but intake and digestion may alter their composition and healthy properties. To asses antioxidants bioavailability at different gastrointestinal-tract levels, in vitro digestion (IvD) simulations are used but reagents involved in may interfere in antioxidant determinations despite depuration procedures are employed.
OBJECTIVE:
To test the magnitude of reagents interference in IvD approaches and the effectiveness of different depuration procedures for reliable antioxidant quantifications on strawberries.
METHODS:
IvD assays were done with water and strawberry samples to obtain digested fractions (gastric and intestinal). After passing-through hydrophilic cotton, digested extracts were subjected to different depuration procedures: centrifugation, Sep-Pack and 0.45 μm nylon-filter. Antioxidant content and capacity were evaluated spectrophotometrically.
RESULTS:
IvD reagents interfered in all antioxidant determinations, especially in the intestinal fractions. Depuration procedures differed in their effectiveness for reagents removal and in their antioxidant retrieval efficiency, with hydrophilic cotton displaying better recovery efficiency.
CONCLUSIONS:
Reagents interference should be considered for antioxidant content and capacity determinations after IvD but, for reliable estimations of healthy compounds of food matrices, depuration methods should prioritize antioxidant recovery over reagents removal.
HannumSM. Potential impact of strawberries on human health: A review of the science. Critical Reviews in Food Science and Nutrition. 2004;44:1–17.
2.
GiampieriF, TulipaniS, Alvarez-SuarezJM, QuilesJL, MezzettiB, BattinoM. The strawberry: Composition, nutritional quality, and impact on human health. Nutrition. 2012;28:9–19.
3.
MeyersKJ, WatkinsCB, PrittsMP, LiuRH. Antioxidant and antiproliferative activities of strawberries. Journal of Agricultural and Food Chemistry. 2003;51:6887–92.
4.
AabyK, SkredeG, WrolstadRE. Phenolic composition and antioxidant activities in flesh and achenes of strawberries (Fragaria ananassa). Journal of Agricultural and Food Chemistry. 2005;53:4032–40.
CarboneF, PreussA, de VosRCH, D’AmicoE, PerrottaG, BovyAR, MartensS, RosatiC. Developmental, genetic and environmental factors affect the expression of flavonoid genes, enzymes and metabolites in strawberry fruits. Plant, Cell & Environment. 2009;32:1117–31.
7.
MezzettiB, BalducciF, CapocasaF, ZhongCF, CappellettiR, Di VittoriL, MazzoniL, GiampieriF, BattinoM. Breeding strawberry for higher phytochemicals content and claim it: Is it possible? International Journal of Fruit Science2016;16:194–206.
8.
Andrianjaka-CampsZN, HeritierJ, AnçayA, AndlauerW, CarlenC. Evolution of the taste-related and bioactive compound profiles of the external and internal tissues of strawberry fruits (Fragaria x ananassa) cv. ‘Clery’ during ripening. Journal of Berry Research. 2017;7:11–22.
9.
ManachC, ScalbertA, MorandC, RémésyC, JiménezL. Polyphenols: Food sources and bioavailability. The American Journal of Clinical Nutrition. 2004;79:727–47.
10.
McGhieTK, WaltonMC. The bioavailability and absorption of anthocyanins: Towards a better understanding. Molecular Nutrition & Food Research. 2007;51:702–13.
11.
Gil-IzquierdoA, ZafrillaP, Tomás-BarberánFA. An in vitro method to simulate phenolic compound release from the food matrix in the gastrointestinal tract. European Food Research and Technology. 2002;214:155–9.
12.
MinekusM, AlmingerM, AlvitoP, BallanceS, BohnT, BourlieuC, CarrièreF, BoutrouR, CorredigM, DupontD, DufourC, EggerL, GoldingM, KarakayaS, KirkhusB, Le FeunteunS, LesmesU, MacierzankaA, MackieA, MarzeS, McClementsDJ, MénardO, RecioI, SantosCN, SinghRP, VegarudGE, WickhamMSJ, WeitschiesW, BrodkorbA. A standardised static in vitro digestion method suitable for food - an international consensus. Food & Function. 2014;5:1113–24.
13.
ArizaMT, Reboredo-RodríguezP, CervantesL, SoriaC, Martínez-FerriE, Gonzalez-BarreiroC, Cancho-GrandeB, BattinoM, Simal-GándaraJ. Bioaccesibility and potential bioavailability of phenolic compounds as a new target for strawberry breeding programs. Food Chemistry. 2018;248:155–65.
14.
McDougallGJ, DobsonP, SmithP, BlakeA, StewartD. Assessing potential bioavailability of raspberry anthocyanins using an in vitro digestion system. Journal of Agricultural and Food Chemistry. 2005;53:5896–904.
15.
SengulH, SurekE, Nilufer-ErdilD. Investigating the effects of food matrix and food components on bioaccessibility of pomegranate (Punicagranatum) phenolics and anthocyanins using an in-vitro gastrointestinal digestion model. Food Research International. 2014;62:1069–79.
16.
ArizaMT, Reboredo-RodríguezP, MazzoniL, Forbes-HernándezTY, GiampieriF, AfrinS, GasparriniM, SoriaC, Martínez-FerriE, BattinoM, MezzettiB. Strawberry achenes are an important source of bioactive compounds for human health. International Journal of Molecular Sciences. 2016;17:1103.
17.
MarmonSK, UndelandI. Effect of alkaline pH-shift processing on in vitro gastrointestinal digestion of herring (Clupea harengus) fillets. Food Chemistry. 2013;138(1), 214–9.
18.
MartiniS, ConteA, TagliazucchiA. Bioaccessibility, bioactivity and cell metabolism of dark chocolate phenolic compounds after in vitro gastro-intestinal digestion. Journal of Functional Foods. 2018;49:424–36.
19.
MarhuendaJ, AlemánMD, Gironés-VilaplanaA, PérezA, CaravacaG, FigueroaF, MuleroJ, ZafrillaP. Phenolic Composition, Antioxidant Activity, and In Vitro Availability of Four Different Berries Journal of Chemistry2016–org/10.1155/2016/5194901.
20.
AnthonyJP, FyfeL, StewartD, McDougallGJ. Differential effectiveness of berry polyphenols as anti-giardial agents. Parasitology. 2011;138:1110–16.
21.
SingletonVL, OrthoferR, Lamuela-RaventósRM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology. 1999;299:152–78.
22.
DewantoV, WuX, AdomKK, LiuRH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of Agricultural and Food Chemistry. 2002;50:3010–4.
23.
GiustiMM, WrolstadRE. Characterization and measurement of anthocyanins by UV-Visible spectroscopy. In:
GiustiMM,
WrolstadRE, editors. Current Protocols in Food Analytical ChemistryJohn Wiley and Sons, Inc., Hoboken. 2001. (F1.2.1.-F1.2.13)
DeLangeRJ, GlazerAN. Bile acids: Antioxidants or enhancers of peroxidation depending on lipid concentration. Archives of Biochemistry and Biophysics. 1990;276:19–25.
Bermúdez-SotoMJ, Tomás-BarberánFA, García-ConesaMT. Stability of polyphenols in chokeberry (Aronia melanocarpa) subjected to in vitro gastric and pancreatic digestion. Food Chemistry. 2007;102:865–74.
28.
KahlonTS, SmithGE. In vitro binding of bile acids by blueberries (Vaccinium spp.), plums (Prunus spp.), prunes (Prunus spp.), strawberries (Fragaria X ananassa), cherries (Malpighia punicifolia), cranberries (Vaccinium macrocarpon) and apples (Malus sylvestris). Food Chemistry. 2007;100:1182–7.
29.
RyanL, PrescottSL. Stability of the antioxidant capacity of twenty-five commercially available fruit juices subjected to an in vitro digestion. International Journal of Food Science & Technology. 2010;45:1191–7.
30.
TavaresL, FigueiraI, MacedoD, McDougallGJ, LeitãoMC, VieiraHLA, StewartD, AlvesPM, FerreiraRB, SantosCN. Neuroprotective effect of blackberry (Rubus s) polyphenols is potentiated after simulated gastrointestinal digestion. Food Chemistry. 2012;131:1443–52.
31.
DaiJ, MumperRJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010;15:7313–52.
32.
PineloM, LaurieF, WaterhouseA. A simple method to separate red wine nonpolymeric and polymeric phenols by solid-phase extraction. Journal of Agricultural and Food Chemistry. 2006;54:2839–44.
