Restricted accessResearch articleFirst published online 2019-08-28
In vitro rooting and acclimatization of Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem: Testing of auxin,spermidine,and gibberellin for overcoming dormancy
Amelanchier alnifolia is an attractive small fruit difficult to propagate by traditional methods. Moreover, this species can be susceptible to dormancy after transplanting to the soil.
OBJECTIVE:
The aim of this work was to evaluate factors that contribute to effective in vitro rooting and acclimatization of micropropagated shoots of Amelanchier alnifolia.
METHODS:
Different auxins, media concentrations, and sprays containing plant growth regulators were tested. The experimental data were treated by analysis of variance.
RESULTS:
1-naphthalene acetic acid (NAA) was superior for rooting compared to indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). Cultivation of shoots on Murashige & Skoog (MS) medium with a half-strength concentration (1/2 MS) led to higher rooting frequencies than on full-strength MS medium. Addition of 1.5 mg l–1 spermidine to cultivation medium did not significantly improve rooting. Gibberellic acid (GA4+7) alone or in combination with 6-benzylaminopurine (BAP) did not effectively break post-rooting dormancy.
CONCLUSION:
The greatest number of actively growing plants was recorded after rooting the shoots on 1/2 MS medium with 1 mg l–1 NAA followed by a spray treatment with 1 mg l–1 BAP. These results are directly applicable for improving rooting efficiency and acclimatization of micropropagated Amelanchier spp. plantlets.
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