Apoptotic Death Induces Aβ Production and Fibril Formation to a Much Larger Extent than Necrotic-Like Death in CGNs

Abstract

In this study we report that apoptotic death of primary cultures of cerebellar granule neurons is accompanied by release of thioflavin-binding proteins – indicative of the presence of β-sheet structures – and fibril formation in the culture medium. When the same neurons are subjected to an excytotoxic death caused by 100 µM glutamate exposure, the amount of thioflavin binding is markedly reduced. Western blot analysis shows that fibrils contain monomers, dimers and trimers of amyloid-β (Aβ) which, when observed at the electron microscope, have morphologies reminiscent of fibrils of senile plaques. These findings demonstrate that triggering an apoptotic pathway leads to β-sheet transition and fibril formation of a protein primarily involved in Alzheimer's disease and may be of direct relevance to the possible link between apoptosis and this neuropathology.