Abstract
Neuritic plaques, one of the diagnostic characteristics of an AD, contain extracellular deposits of amyloid-β (Aβ) derived from amyloid-β protein precursor (AβPP). The objective of this study was to extract AβPP out of HEK293 cells and to purify it. Two procedures were chosen for purification of AβPP: Thiophilic Interaction Chromatography (TIC) and molecular sieving. Using Superdex 75, Superose 12, and Fractogel gel matrices, AβPP was isolated on HPLC. The chromatograms illustrate the purification of AβPP. Our method describes a new and elegant way for the extraction and purification of AβPP from HEK293 cell lines using thiophilic interaction chromatography (TIC).
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