Abstract
Background: Age and short leukocyte telomeres have been associated with a
higher risk of Alzheimer’s disease (AD). Inflammation is involved in AD and it is
suggested that anti-inflammatory interleukin-10 (IL-10) may partly antagonize these
processes.
Objective: The aim is to correlate telomere length (TL) in peripheral blood
mononuclear cells (PBMC) from patients with AD to disease progression rate. Moreover, we
evaluated whether TL was associated with IL-10 production by unstimulated or amyloid-β
(Aβ)-stimulated PBMC.
Methods: We enrolled 31 late-onset AD and 20 age-matched healthy elderly
(HE). After a two-year follow-up period, patients were retrospectively evaluated as
slow-progressing (ADS) (Mini Mental State Examination (MMSE) decline over the two years of
follow-up ≤3 points) or fast progressing AD (ADF) (MMSE decline ≥5 points). TL was
measured by flow cytometry and in vitro IL-10 production by enzyme-linked
immunosorbent assay.
Results: TL (mean±SD) for HE, ADS, and ADF was 2.3±0.1, 2.0±0.1, and 2.5±0.1
Kb, respectively. ADS showed a shorter TL compared to HE (p = 0.034) and
to ADF (p = 0.005). MMSE decline correlated with TL in AD
(R2 = 0.284; p = 0.008). We found a significant difference in
IL-10 production between unstimulated and Aβ-stimulated PBMC from ADS (40.7±13.7 versus
59.0±27.0; p = 0.004) but not from ADF (39.7±14.4 versus 42.2±22.4). HE
showed a trend toward significance (47.1±25.4 versus 55.3±27.9;
p = 0.10).
Conclusion: PBMC from ADF may be characterized by an impaired response
induced by Aβ and by a reduced proliferative response responsible for the longer
telomeres. TL might be a contributing factor in predicting the rate of AD progression.
