Endothelin-Converting Enzyme-1 Activity,Endothelin-1 Production,and Free Radical-Dependent Vasoconstriction in Alzheimer's Disease

Abstract

Alzheimer's disease (AD) patients have reduced cerebral blood flow. This precedes dementia and may contribute to its progression. In mice that overexpress amyloid-β protein precursor, cerebral blood flow declines before the development of plaques or cognitive abnormalities. In the brain, endothelin-1 (ET-1) is a locally acting vasoconstrictor, produced in neurons by endothelin-converting enzyme (ECE)-2 and in endothelial cells by ECE-1. Both ECEs are also capable of cleaving amyloid-β (Aβ). We previously showed ECE-2 and ET-1 to be elevated in postmortem temporal cortex from AD patients, and ECE-2 expression and ET-1 release to be upregulated by Aβ₄₂ in vitro. We have now studied isolated leptomeningeal blood vessels from postmortem brains and found that although ECE-1 level is reduced, ECE-1 activity and ET-1 level are significantly elevated in AD vessels. This is specific to AD as there is no specific change in vascular dementia vessels. In primary cultures of human brain endothelial cells, both Aβ₄₀ and Aβ₄₂ caused a significant increase in ET-1 release, the increase being particularly pronounced with Aβ₄₀. In view of previous studies implicating free radicals in the endothelial dysfunction caused by Aβ₄₀, we examined whether Aβ-mediated ET-1 release could be prevented by the antioxidant superoxide dismutase. Addition of superoxide dismutase to cells exposed to Aβ₄₀ prevented the increase in the concentration of ET-1. Our findings indicate that cerebral vasoconstriction induced by Aβ results in part from a free radical-mediated increase in ECE-1 activity and ET-1 production.