Abstract
We have combined the molecular biology methods of the polymerase chain reaction and recombinant DNA cloning in bacteriophage fambda to express a human IgM Fab in Escherichia coli using genes derived from an Epstein-Barr vzrus transformed cell line. This method comprises three cDNA amplifications and a single clonzng step, culminating In the stable overexpression of mammalian heterodimeric recombinant protein in a prokaryotic host.
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